应用青霉素结合蛋白 2a 乳胶凝集试验直接检测血培养肉汤中的耐甲氧西林金黄色葡萄球菌。
Direct detection of methicillin resistance in Staphylococcus aureus in blood culture broth by use of a penicillin binding protein 2a latex agglutination test.
机构信息
Division of Laboratory and Transfusion Medicine, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
出版信息
J Clin Microbiol. 2010 Apr;48(4):1420-1. doi: 10.1128/JCM.01517-09. Epub 2010 Feb 3.
Abstract
We studied the utility of performing a penicillin binding protein 2a latex agglutination (PBP-LA) assay directly on Bactec blood culture broth samples containing Staphylococcus aureus to rapidly detect methicillin resistance. The sensitivity, specificity, positive predictive value, and negative predictive value of this method were 94.1%, 97.5%, 98%, and 92.9%, respectively.
摘要
我们研究了在含有金黄色葡萄球菌的 Bactec 血培养肉汤样本上直接进行青霉素结合蛋白 2a 乳胶凝集（PBP-LA）检测以快速检测耐甲氧西林的实用性。该方法的灵敏度、特异性、阳性预测值和阴性预测值分别为 94.1%、97.5%、98%和 92.9%。
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