Gardner Robert D, Zhou Anhong, Zufelt Nephi A
Department of Biological and Irrigation Engineering, Utah State University, 4105 Old Main Hill, Logan, Utah 84322-4105, U.S.A.
Sens Actuators B Chem. 2009 Feb 2;136(1):177-185. doi: 10.1016/j.snb.2008.10.031.
A microelectrode array sensor platform was designed and fabricated to increase diversity, flexibility, and versatility of testing capabilities over that of traditionally reported sensor platforms. These new sensor platforms consist of 18 individual addressable microelectrodes, photolithography fabricated, that employ a glass base substrate and a resist polymer layer that acts as an insulating agent to protect the circuitry and wiring of the sensor from undesired solution interactions. Individually addressable microelectrodes increase diversity by allowing isolated electrochemical testing between electrodes, global array testing, or some combination of electrodes to perform electrochemical methods. Furthermore, because of the optical transparency of the glass base substrate and the resist mask layer, along with the small size of the electrode array, spectrochemical analysis is possible within the sample area that acts as electrochemical cell and cuvette, while the microelectrode array passively resides within the optical path length during spectrochemical testing. This unique arrangement offers improved testing possibilities for various applications, including simultaneous electrochemical and spectrochemical analysis in environmental testing, identification or quantification of possible species for bioavailability in the biotechnology field, and process control in industrial applications. Electrochemical characteristics and spectrochemcial use of the sensor platform are proven with potassium ferricyanide, an electrochemical standard analyte, and electrochemical measurements are compared against a commercially available working electrode of similar size. Additionally, the electrochemical method of differential pulse anodic stripping voltammetry is performed with the sensor platform to detect copper and lead heavy metal ions in aqueous solution, demonstrating the potential for use with environmental samples.
设计并制造了一种微电极阵列传感器平台，以提高测试能力的多样性、灵活性和通用性，超越传统报道的传感器平台。这些新的传感器平台由18个独立可寻址的微电极组成，通过光刻制造，采用玻璃基底和抗蚀剂聚合物层，该聚合物层作为绝缘剂，保护传感器的电路和布线免受不必要的溶液相互作用。独立可寻址的微电极通过允许电极之间的孤立电化学测试、全局阵列测试或电极的某种组合来执行电化学方法，从而增加了多样性。此外，由于玻璃基底和抗蚀剂掩膜层的光学透明性，以及电极阵列的小尺寸，在用作电化学池和比色皿的样品区域内可以进行光谱化学分析，而在光谱化学测试期间，微电极阵列被动地位于光路长度内。这种独特的布置为各种应用提供了更好的测试可能性，包括环境测试中的同时电化学和光谱化学分析、生物技术领域中生物可利用性可能物种的鉴定或定量，以及工业应用中的过程控制。使用电化学标准分析物铁氰化钾证明了传感器平台的电化学特性和光谱化学用途，并将电化学测量结果与类似尺寸的市售工作电极进行了比较。此外，使用传感器平台进行差分脉冲阳极溶出伏安法的电化学方法来检测水溶液中的铜和铅重金属离子，证明了其用于环境样品的潜力。
