Wang Guey-Horng, Chou Tzung-Han, Lin Rong-Jyh, Sheu Jyh-Horng, Wang Shih-Hao, Liang Chia-Hua
Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan.
J Toxicol. 2009;2009:634868. doi: 10.1155/2009/634868. Epub 2008 Oct 13.
Soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. grandilobata, S. parva, S. triangula, S. scabra, S. nanolobata and S. gibberosa) by SCC25 and HaCaT cells. The cell adhesion assay indicates that extracts reduce the cell attachment. Extracts exhibit a dose-dependent cytotoxic effect using MTS assay.Treatment of extracts to observe the morphological alterations in cells, membrane blebbing, nuclear condensation, and apoptotic bodies is demonstrated. Flow cytometry shows that extracts sensitized the cells in the G(0)/G(1) and G(2)/M phases with a concomitant significantly increased sub-G(1) fraction, suggesting cell death by apoptosis. Extracts of the genus Sinularia thus apparently cause apoptosis of SCC25 and HaCaT cells, and warrant further research investigating the possible antioral cancer compounds in these soft corals.
肉芝软珊瑚属的软珊瑚正越来越多地被用于治疗多种疾病过程。然而，其对人类口腔癌细胞的作用机制却知之甚少。本研究评估了肉芝软珊瑚属提取物（大裂片肉芝软珊瑚、小叶肉芝软珊瑚、三角肉芝软珊瑚、粗糙肉芝软珊瑚、纳米肉芝软珊瑚和瘤肉芝软珊瑚）对SCC25和HaCaT细胞的细胞毒性作用。细胞黏附试验表明提取物可减少细胞黏附。使用MTS试验，提取物表现出剂量依赖性细胞毒性作用。通过对提取物进行处理来观察细胞中的形态学改变，如细胞膜起泡、核浓缩和凋亡小体。流式细胞术显示提取物使处于G(0)/G(1)和G(2)/M期的细胞敏感化，同时亚G(1)期部分显著增加，表明细胞通过凋亡死亡。因此，肉芝软珊瑚属提取物显然会导致SCC25和HaCaT细胞凋亡，值得进一步研究这些软珊瑚中可能的抗口腔癌化合物。
