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原肌球蛋白周期3对于增强细肌丝重构牛心肌中的等长张力至关重要。

Tropomyosin period 3 is essential for enhancement of isometric tension in thin filament-reconstituted bovine myocardium.

作者信息

Kawai Masataka, Lu Xiaoying, Hitchcock-Degregori Sarah E, Stanton Kristen J, Wandling Michael W

机构信息

Department of Anatomy and Cell Biology, The University of Iowa, Iowa City, IA 52242, USA.

出版信息

J Biophys. 2009;2009:380967. doi: 10.1155/2009/380967. Epub 2009 Oct 13.

DOI:10.1155/2009/380967
PMID:20130792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2814127/
Abstract

Tropomyosin (Tm) consists of 7 quasiequivalent repeats known as "periods," and its specific function may be associated with these periods. To test the hypothesis that either period 2 or 3 promotes force generation by inducing a positive allosteric effect on actin, we reconstituted the thin filament with mutant Tm in which either period 2 (Delta2Tm) or period 3 (Delta3Tm) was deleted. We then studied: isometric tension, stiffness, 6 kinetic constants, and the pCa-tension relationship. N-terminal acetylation of Tm did not cause any differences. The isometric tension in Delta2Tm remained unchanged, and was reduced to approximately 60% in Delta3Tm. Although the kinetic constants underwent small changes, the occupancy of strongly attached cross-bridges was not much different. The Hill factor (cooperativity) did not differ significantly between Delta2Tm (1.79 +/- 0.19) and the control (1.73 +/- 0.21), or Delta3Tm (1.35 +/- 0.22) and the control. In contrast, pCa(50) decreased slightly in Delta2Tm (5.11 +/- 0.07), and increased significantly in Delta3Tm (5.57 +/- 0.09) compared to the control (5.28 +/- 0.04). These results demonstrate that, when ions are present at physiological concentrations in the muscle fiber system, period 3 (but not period 2) is essential for the positive allosteric effect that enhances the interaction between actin and myosin, and increases isometric force of each cross-bridge.

摘要

原肌球蛋白（Tm）由7个被称为“周期”的准等效重复序列组成，其特定功能可能与这些周期相关。为了验证周期2或周期3通过对肌动蛋白产生正变构效应来促进力产生的假设，我们用缺失周期2（Delta2Tm）或周期3（Delta3Tm）的突变型Tm重构了细肌丝。然后我们研究了：等长张力、刚度、6个动力学常数以及pCa-张力关系。Tm的N端乙酰化没有引起任何差异。Delta2Tm中的等长张力保持不变，而Delta3Tm中的等长张力降低到约60%。尽管动力学常数有小的变化，但强附着横桥的占有率没有太大差异。Delta2Tm（1.79±0.19）与对照组（1.73±0.21）之间或Delta3Tm（1.35±0.22）与对照组之间的希尔系数（协同性）没有显著差异。相比之下，与对照组（5.28±0.04）相比，Delta2Tm中的pCa(50)略有下降（5.11±0.07），而Delta3Tm中的pCa(50)显著增加（5.57±0.09）。这些结果表明，当肌肉纤维系统中离子浓度处于生理浓度时，周期3（而非周期2）对于增强肌动蛋白和肌球蛋白之间相互作用并增加每个横桥等长力的正变构效应至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/b64c91d400c7/JBP2009-380967.011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/d8a6f3370541/JBP2009-380967.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/78427dbb02b6/JBP2009-380967.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/b8217b96410a/JBP2009-380967.006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/c41d3d38188b/JBP2009-380967.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/1af18557f317/JBP2009-380967.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/7cf4232e0646/JBP2009-380967.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/b64c91d400c7/JBP2009-380967.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/b8314106bf4e/JBP2009-380967.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/69c64d2c05de/JBP2009-380967.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/999eb17b12ce/JBP2009-380967.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/d35ff9d9a1e6/JBP2009-380967.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/d8a6f3370541/JBP2009-380967.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/78427dbb02b6/JBP2009-380967.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/b8217b96410a/JBP2009-380967.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/f92297874698/JBP2009-380967.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/c41d3d38188b/JBP2009-380967.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/1af18557f317/JBP2009-380967.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/7cf4232e0646/JBP2009-380967.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdc/2814127/b64c91d400c7/JBP2009-380967.011.jpg

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2
Force transients and minimum cross-bridge models in muscular contraction.肌肉收缩中的力瞬变与最小横桥模型
J Muscle Res Cell Motil. 2007;28(7-8):371-95. doi: 10.1007/s10974-008-9131-3. Epub 2008 Apr 19.
3
Temperature-dependence of isometric tension and cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutant.
Biophys Physicobiol. 2019 Feb 2;16:28-40. doi: 10.2142/biophysico.16.0_28. eCollection 2019.
4
Transmission of stability information through the N-domain of tropomyosin is interrupted by a stabilizing mutation (A109L) in the hydrophobic core of the stability control region (residues 97-118).稳定性信息通过原肌球蛋白 N 结构域传递，稳定性控制区域（残基 97-118）的疏水核心的稳定突变（A109L）会中断这种传递。
J Biol Chem. 2014 Feb 14;289(7):4356-66. doi: 10.1074/jbc.M113.507236. Epub 2013 Dec 20.
5
Periodicities designed in the tropomyosin sequence and structure define its functions.原肌球蛋白序列和结构中设计的周期性决定了它的功能。
Bioarchitecture. 2013 May-Jun;3(3):51-6. doi: 10.4161/bioa.25616. Epub 2013 Jul 8.
6
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7
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PLoS One. 2012;7(10):e47471. doi: 10.1371/journal.pone.0047471. Epub 2012 Oct 15.
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4
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J Muscle Res Cell Motil. 2006;27(5-7):455-68. doi: 10.1007/s10974-006-9075-4. Epub 2006 Aug 15.
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8
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9
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10
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