测定生长大鼠对磷虾蛋白浓缩物中ω-3 脂肪酸含量的消化率、组织沉积和代谢。

Determination of digestibility, tissue deposition, and metabolism of the omega-3 fatty acid content of krill protein concentrate in growing rats.

机构信息

Human Nutrition and Foods, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, West Virginia 26506, USA.

出版信息

J Agric Food Chem. 2010 Mar 10;58(5):2830-7. doi: 10.1021/jf9036408.

DOI:10.1021/jf9036408

PMID:20131797

Abstract

Krill protein concentrate (KPC) consists of high-quality protein (77.7% dry basis) and lipids (8.1% dry basis) that are rich (27% of total fatty acids) in omega-3 polyunsaturated fatty acids (omega-3 PUFAs). The objective of the study was to determine digestibility, tissue deposition, metabolism, and tissue oxidative stability of the omega-3 PUFAs provided by KPC. Young female Sprague-Dawley rats (n = 10/group) were fed ad libitum isocaloric diets for 4 weeks with either 10% freeze-dried KPC or 10% casein. The casein diet contained 5.3% added corn oil (CO), whereas the KPC contained 5.3% total lipids from 0.9% krill oil (KO) provided by KPC and 4.4% added corn oil (KO + CO). Fatty acid compositions of various tissues were analyzed by gas chromatography. Lipid peroxidation was determined by thiobarbituric acid reactive substances (TBARS). Total antioxidant capacity and urinary eicosanoid metabolites were determined by enzyme immunoassay. The omega-3 PUFAs provided in KO from KPC increased (P = 0.003) docosahexaenoic acid (DHA) concentration in the brain. DHA and eicosapentaenoic acid (EPA) content in fat pads and liver were increased (P < 0.01), whereas the omega-6 PUFA, arachidonic acid (AA), was decreased (P < 0.01) in rats fed the KPC diet containing the KO + CO mixture compared to rats fed the casein diet containing pure CO. Feeding the KPC diet decreased pro-inflammatory 2-series prostaglandin and thromboxane metabolites. There was no significant difference in TBARS or total antioxidant capacity in the tissues of rats fed the different diets. On the basis of the study results, the low amount of omega-3 PUFAs provided by the KO content of KPC provides beneficial effects of increasing tissue EPA and DHA deposition and reduced AA-derived 2-series eicosanoid metabolites without increasing lipid peroxidation. Therefore, consumption of KPC has the potential to provide a healthy and sustainable source of omega-3 PUFAs.

摘要

磷虾蛋白浓缩物（KPC）由高质量的蛋白质（干基 77.7%）和脂质（干基 8.1%）组成，这些脂质富含（总脂肪酸的 27%）ω-3 多不饱和脂肪酸（ω-3 PUFAs）。本研究的目的是确定 KPC 提供的 ω-3 PUFAs 的消化率、组织沉积、代谢和组织氧化稳定性。10 只/组的年轻雌性 Sprague-Dawley 大鼠自由摄取等热量饮食 4 周，分别用 10%冷冻干燥的 KPC 或 10%酪蛋白喂养。酪蛋白饮食中添加了 5.3%玉米油（CO），而 KPC 中含有 5.3%来自 KPC 的 0.9%磷虾油（KO）的总脂质和 4.4%添加的玉米油（KO+CO）。通过气相色谱法分析各种组织的脂肪酸组成。通过硫代巴比妥酸反应物质（TBARS）测定脂质过氧化。通过酶免疫测定法测定总抗氧化能力和尿类二十烷酸代谢物。KPC 中的 KO 提供的 ω-3 PUFAs 增加了（P=0.003）大脑中的二十二碳六烯酸（DHA）浓度。脂肪垫和肝脏中的 DHA 和二十碳五烯酸（EPA）含量增加（P<0.01），而摄入 KPC 饮食中含有 KO+CO 混合物的大鼠的 ω-6 多不饱和脂肪酸，花生四烯酸（AA）含量降低（P<0.01），与摄入含有纯 CO 的酪蛋白饮食的大鼠相比。饲喂 KPC 饮食可降低促炎 2 系列前列腺素和血栓素代谢物。不同饮食喂养的大鼠组织中的 TBARS 或总抗氧化能力无显著差异。根据研究结果，KPC 的 KO 含量提供的少量 ω-3 PUFAs 具有增加组织 EPA 和 DHA 沉积和减少 AA 衍生的 2 系列类二十烷酸代谢物的有益作用，而不会增加脂质过氧化。因此，摄入 KPC 有可能提供健康和可持续的 ω-3 PUFAs 来源。