在模拟胃肠道条件下携带丙型肝炎病毒衍生表位的黄瓜花叶病毒纳米颗粒的体外稳定性和口服疫苗的体内功效。
In vitro stability of Cucumber mosaic virus nanoparticles carrying a Hepatitis C virus-derived epitope under simulated gastrointestinal conditions and in vivo efficacy of an edible vaccine.
机构信息
Department of Biology, Plant Protection and Agrobiotechnology, University of Basilicata, 85100 Potenza, Italy.
出版信息
J Virol Methods. 2010 May;165(2):211-5. doi: 10.1016/j.jviromet.2010.01.021. Epub 2010 Feb 2.
The Cucumber mosaic virus (CMV) is an isodiametric plant virus with an extremely wide host range, present worldwide. CMV chimeric particles (R9-CMV), engineered to express a 27-aa synthetic peptide derived from Hepatitis C virus (HCV), were demonstrated to be stable under simulated gastric and intestinal conditions. Then the possibility of inducing a humoral immune response in rabbits fed with R9-CMV infected lettuce plants was demonstrated, suggesting that this system could function as a confirming tool of a bioreactor for the production of a stable edible vaccine against HCV.
黄瓜花叶病毒（CMV）是一种等径植物病毒，具有极其广泛的宿主范围，存在于世界各地。表达源自丙型肝炎病毒（HCV）的 27 个氨基酸合成肽的 CMV 嵌合颗粒（R9-CMV）在模拟胃和肠道条件下表现出稳定。然后证明了在用感染了 R9-CMV 的生菜喂养的兔子中诱导体液免疫反应的可能性，这表明该系统可以作为生产稳定的 HCV 口服疫苗的生物反应器的确认工具。
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