Department of Biology, Plant Protection and Agrobiotechnology, University of Basilicata, 85100 Potenza, Italy.
J Virol Methods. 2010 May;165(2):211-5. doi: 10.1016/j.jviromet.2010.01.021. Epub 2010 Feb 2.
The Cucumber mosaic virus (CMV) is an isodiametric plant virus with an extremely wide host range, present worldwide. CMV chimeric particles (R9-CMV), engineered to express a 27-aa synthetic peptide derived from Hepatitis C virus (HCV), were demonstrated to be stable under simulated gastric and intestinal conditions. Then the possibility of inducing a humoral immune response in rabbits fed with R9-CMV infected lettuce plants was demonstrated, suggesting that this system could function as a confirming tool of a bioreactor for the production of a stable edible vaccine against HCV.
黄瓜花叶病毒（CMV）是一种等径植物病毒，具有极其广泛的宿主范围，存在于世界各地。表达源自丙型肝炎病毒（HCV）的 27 个氨基酸合成肽的 CMV 嵌合颗粒（R9-CMV）在模拟胃和肠道条件下表现出稳定。然后证明了在用感染了 R9-CMV 的生菜喂养的兔子中诱导体液免疫反应的可能性，这表明该系统可以作为生产稳定的 HCV 口服疫苗的生物反应器的确认工具。
