在内脏利什曼病流行地区，用利什曼疫苗接种的犬只出现CD4+/CD25+ T细胞减少和干扰素-γ水平升高的情况。

Diminished CD4+/CD25+ T cell and increased IFN-gamma levels occur in dogs vaccinated with Leishmune in an endemic area for visceral leishmaniasis.

作者信息

de Lima Valéria Marçal Felix, Ikeda Fabiana Augusta, Rossi Cláudio N, Feitosa Mary Marcondes, Vasconcelos Rosemeride Oliveira, Nunes Caris Maroni, Goto Hiro

机构信息

Departamento de Clínica, Cirugia e Reproduçãp Animal, Faculdade de Odontologia, Curso de Medicina Veterinária, Universidade Estadual Paulista Júlio de Mesquita Filho, Araçatuba Campus, SP, Brazil.

出版信息

Vet Immunol Immunopathol. 2010 Jun 15;135(3-4):296-302. doi: 10.1016/j.vetimm.2009.12.008. Epub 2010 Jan 21.

DOI:10.1016/j.vetimm.2009.12.008

PMID:20132994

Abstract

The Leishmune vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in Brazil, but cytokine production induced by vaccination has rarely been investigated in dogs. This study aimed to evaluate the immune response of dogs vaccinated with Leishmune FML vaccine (Fort Dodge) against total antigen of Leishmania (Leishmania) chagasi (TAg) and FML. Twenty healthy dogs from Araçatuba, São Paulo, Brazil, an endemic leishmaniasis area, received three consecutive subcutaneous injection of Leishmune vaccine at 21-day intervals. PBMC were isolated before and 10 days after completing vaccination and lymphoproliferative response and antibody production against FML or total promastigote antigen were tested. Cytokines IFN-gamma, IL-4 and TNF-alpha were measured in culture supernatant and CD4+/CD25+ and CD8+/CD25+ T cell presence was determined. Analysis of the data indicated that the vaccine conferred humoral responses (100%) against both antigens and cellular immunity to FML (85%) and total antigen (80%), the supernatant of cultured cells stimulated with TAg and FML showed an increase in IFN-gamma (P<0.05), and the vaccine reduced CD4+/CD25+ T cell presence compared to that observed before vaccination. These responses may constitute part of the immune mechanism induced by Leishmune.

摘要

利什曼疫苗已在流行地区用于预防巴西犬内脏利什曼病，但在犬类中很少研究疫苗接种诱导的细胞因子产生情况。本研究旨在评估接种利什曼FML疫苗（辉瑞动物保健公司）的犬对恰加斯利什曼原虫（Leishmania chagasi）总抗原（TAg）和FML的免疫反应。来自巴西圣保罗州阿拉萨图巴（一个利什曼病流行地区）的20只健康犬，每隔21天连续进行三次皮下注射利什曼疫苗。在完成疫苗接种前及接种后10天分离外周血单核细胞（PBMC），检测其对FML或前鞭毛体总抗原的淋巴细胞增殖反应和抗体产生情况。检测培养上清液中的细胞因子γ干扰素（IFN-γ）、白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α），并确定CD4⁺/CD25⁺和CD8⁺/CD25⁺ T细胞的存在情况。数据分析表明，该疫苗对两种抗原均产生了体液反应（100%），对FML（85%）和总抗原（80%）产生了细胞免疫，用TAg和FML刺激的培养细胞上清液中IFN-γ增加（P<0.05），与接种前相比，该疫苗降低了CD4⁺/CD25⁺ T细胞的存在。这些反应可能构成利什曼疫苗诱导的免疫机制的一部分。