Alpha-haemolysin from E. coli. Purification and self-aggregation properties.
作者信息
Ostolaza H, Bartolomé B, Serra J L, de la Cruz F, Goñi F M
机构信息
Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
出版信息
FEBS Lett. 1991 Mar 25;280(2):195-8. doi: 10.1016/0014-5793(91)80291-a.
PMID:2013312
Abstract
An improved, straightforward purification procedure for E.coli alpha-haemolysin has been developed. The protein exists in the form of large aggregates, held together mainly by hydrophobic forces. In the presence of urea or other chaotropic agents, the size of the aggregates decreases, while the specific activity is increased.
摘要
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