Affranchino J L, Pollevick G D, Frasch A C
Instituto de Investigaciones Bioquímicas Fundación Campomar, Avenida Patricias Argentina, Buenos Aires.
FEBS Lett. 1991 Mar 25;280(2):316-20. doi: 10.1016/0014-5793(91)80320-3.
To better understand the mechanisms involved in the developmental expression of Trypanosoma cruzi antigens we examined the gene structure and transcription properties of the major shed trypomastigote antigen (SAPA). We report in this paper that SAPA is encoded by a small family of at lest 6 genes which differ mainly in the length of a repeat region made up of tandemly arranged 36-bp repeat units. SAPA genes are located distant from chromosomal telomeres as inferred from their insensitivity to Bal31 nuclease treatment. Furthermore, Northern blot and S1 protection analyses strongly support the fact that most (or all) SAPA genes are transcribed in the infective form of the parasite.
为了更好地理解克氏锥虫抗原发育表达所涉及的机制，我们研究了主要脱落型锥鞭毛体抗原（SAPA）的基因结构和转录特性。我们在本文中报告，SAPA由一个至少包含6个基因的小家族编码，这些基因主要区别在于由串联排列的36 bp重复单元组成的重复区域的长度。从其对Bal31核酸酶处理不敏感推断，SAPA基因位于远离染色体端粒的位置。此外，Northern印迹和S1核酸酶保护分析有力地支持了大多数（或全部）SAPA基因在寄生虫感染形式中被转录这一事实。
