Institute for Prevention and Occupational Medicine, German Social Accident Insurance-Institute, Ruhr-University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, Bochum, Germany.
Hum Exp Toxicol. 2010 Sep;29(9):721-9. doi: 10.1177/0960327109359635. Epub 2010 Feb 4.
DNA strand breaks were determined in leucocytes of induced sputum (IS) and compared with DNA strand breaks in blood lymphocytes from 42 bitumen-exposed workers pre and post shift. Comet assay results were expressed in arbitrary units based on visual scoring (sputum leucocytes) and Olive tail moment (OTM, blood lymphocytes). DNA damage in IS leucocytes was overall high but did not change during shift. Level of DNA strand breaks in IS samples correlated with total cell count and neutrophil content (Spearman rank correlation coefficient r(s) = 0.47, p = 0.001, r(s)= 0.48, p = 0.001, respectively) and with IL-8 concentration before and after shift (r(s) = 0.31, P = 0.048, and r(s) = 0.43, P = 0.005). DNA damage in IS was not associated with DNA strand breaks in blood lymphocytes (r(s) = -0.04, p = 0.802 before shift, r(s) = 0.27, p = 0.088 after shift). A higher level of DNA strand breaks was measured in blood lymphocytes before shift (median OTM 1.7 before and 1.3 after shift, p = 0.023). A strong correlation was found between the number of neutrophils and IL-8 concentration in IS before and after shift (r(s) = 0.77 and r(s)= 0.75, p < 0.001). This study showed an association between genotoxic and inflammatory effects in the lower airways and compared simultaneously DNA strand breaks in IS and blood of bitumen-exposed workers.
白细胞 DNA 链断裂在诱导痰（IS）中被确定，并与 42 名沥青暴露工人工作前后血液淋巴细胞中的 DNA 链断裂进行比较。彗星试验结果以基于视觉评分（痰白细胞）和 Olive 尾巴矩（OTM，血液淋巴细胞）的任意单位表示。IS 白细胞中的 DNA 损伤总体较高，但在轮班过程中没有变化。IS 样本中 DNA 链断裂的水平与总细胞计数和中性粒细胞含量相关（Spearman 秩相关系数 r(s) = 0.47，p = 0.001，r(s) = 0.48，p = 0.001），与轮班前和轮班后的 IL-8 浓度相关（r(s) = 0.31，P = 0.048，和 r(s) = 0.43，P = 0.005）。IS 中的 DNA 损伤与血液淋巴细胞中的 DNA 链断裂无关（r(s) = -0.04，p = 0.802 轮班前，r(s) = 0.27，p = 0.088 轮班后）。在轮班前测量到血液淋巴细胞中的 DNA 链断裂水平更高（中位数 OTM 轮班前为 1.7，轮班后为 1.3，p = 0.023）。在轮班前和轮班后 IS 中，中性粒细胞数量和 IL-8 浓度之间存在很强的相关性（r(s) = 0.77 和 r(s) = 0.75，p < 0.001）。本研究显示了下呼吸道遗传毒性和炎症效应之间的关联，并同时比较了沥青暴露工人 IS 和血液中的 DNA 链断裂。
