Fuchs J, Hengstler J G, Boettler G, Oesch F
Institute of Toxicology, University of Mainz, Germany.
Int Arch Occup Environ Health. 1996;68(3):141-6. doi: 10.1007/BF00381622.
The genotoxic effect of occupational exposure to bitumen-based products was determined by the extent of DNA strand breaks and alkali-labile sites of the DNA of peripheral mononuclear blood cells from seven roofers, 18 road paving workers, and nine bitumen painters. In order to evaluate short-term genotoxic effect the workers were investigated on Fridays and on Mondays after a weekend free of occupational exposure. The roofers (all cigarette smokers) showed a significantly (P < 0.002) 43% higher mean level of alkaline DNA strand breaks on Friday than did the ten smoking controls included in this study. Also, comparison of the individual levels of alkaline strand breaks on Mondays and on Fridays revealed a significant increase (P < 0.05, Wilcoxon test) during the work week. In the road paving workers and the bitumen painters no statistically significant difference in the mean levels of alkaline strand breaks could be found compared to controls either for the measurement on Mondays or for that on Fridays. However, interesting tendencies were observed. As in the group of roofers, the mean level of alkaline DNA strand breaks as well as the majority of the individual levels of alkaline strand breaks of road paving workers was higher on Fridays than on Mondays. In contrast, bitumen painters exhibited a relatively high level of alkaline DNA strand breaks on Mondays and a decreased mean level of strand breaks on Fridays. DNA adducts could be detected at a low level (up to 2.9 adducts per 10(9) bases) in 10 of 14 road paving workers and bitumen painters using the 32P-postlabelling assay. The number of DNA adducts correlated with the years spend in the present job. Road paving workers and bitumen painters showed only suggestive evidence for a possible genotoxic effect due to their occupational exposure. Because we cannot exclude the formation of DNA cross-links in these workers, a more detailed investigation of the hazard is urgently needed. For roofers, substantial genotoxic damage in peripheral mononuclear blood cells was observed in this study.
通过检测7名屋顶工、18名道路铺设工人和9名沥青漆工外周血单个核细胞DNA链断裂程度以及DNA碱不稳定位点，来确定职业性接触沥青基产品的遗传毒性效应。为评估短期遗传毒性效应，在工人周末无职业接触后的周五和周一进行调查。屋顶工（均为吸烟者）周五的碱性DNA链断裂平均水平比本研究纳入的10名吸烟对照者显著高43%（P<0.002）。此外，比较周一和周五个体的碱性链断裂水平发现，工作日期间有显著增加（P<0.05，Wilcoxon检验）。道路铺设工人和沥青漆工的碱性链断裂平均水平，与对照相比，无论是周一还是周五的测量结果，均未发现统计学上的显著差异。然而，观察到了有趣的趋势。与屋顶工组一样，道路铺设工人周五的碱性DNA链断裂平均水平以及大多数个体的碱性链断裂水平高于周一。相比之下，沥青漆工周一的碱性DNA链断裂水平相对较高，周五的链断裂平均水平降低。使用32P后标记分析法，在14名道路铺设工人和沥青漆工中的10人身上检测到低水平的DNA加合物（每109个碱基最多2.9个加合物）。DNA加合物的数量与从事当前工作的年限相关。道路铺设工人和沥青漆工仅显示出职业接触可能导致遗传毒性效应的提示性证据。由于我们不能排除这些工人中DNA交联的形成，因此迫切需要对这种危害进行更详细的调查。在本研究中观察到屋顶工外周血单个核细胞有大量遗传毒性损伤。
