Faculty of Science, Josai University, Sakado, Saitama 350-0295, Japan.
In Vivo. 2010 Jan-Feb;24(1):39-44.
We have recently reported that out of twenty benzo[b]cyclohept[e][1,4]oxazines and their S-analogs, and 2-aminotropone derivatives, 7-bromo-2-(4-hydroxyanilino) tropone and 4-isopropyl-2-(2-hydroxyanilino)tropone showed the highest tumor-specificity in human oral squamous cell carcinoma cell lines. To gain more insight into the anti-tumor actions of these compounds, whether they induce the growth stimulation effect observed at low concentrations, known as hormesis, was investigated using a total of ten human normal and tumor cultured cells. The tumor-specificity of both compounds became apparent 48 hours after the start of treatment of the cells with these compounds and reached a maximum level at 72 and 96 hours. On the other hand, their growth stimulatory effects were most prominent at 24 hours, especially in normal skin and lung fibroblasts, but rapidly disappeared with prolonged incubation time (48-96 hours). These data suggest the occurrence of a hormetic response only at restricted times and concentrations as has been previously reported, although the biological significance is yet to be elucidated.
我们最近报道，在二十个苯并[b]环庚[e][1,4]恶嗪及其 S-类似物和 2-氨基-1-酮衍生物中，7-溴-2-(4-羟基苯胺基) -1-酮和 4-异丙基-2-(2-羟基苯胺基)-1-酮在人口腔鳞状细胞癌细胞系中显示出最高的肿瘤特异性。为了更深入地了解这些化合物的抗肿瘤作用，我们用总共十种人正常和肿瘤培养细胞研究了它们是否诱导低浓度下观察到的生长刺激效应，即毒物兴奋效应。这两种化合物的肿瘤特异性在开始用这些化合物处理细胞后 48 小时变得明显，并在 72 和 96 小时达到最大值。另一方面，它们的生长刺激作用在 24 小时最为明显，尤其是在正常皮肤和肺成纤维细胞中，但随着孵育时间的延长（48-96 小时），这种作用迅速消失。这些数据表明，尽管其生物学意义尚待阐明，但正如先前报道的那样，仅在特定时间和浓度范围内会发生毒物兴奋反应。
