Faculty of Science, Josai University, Sakado, Saitama 350-0295, Japan.
In Vivo. 2010 Nov-Dec;24(6):843-51.
We have previously investigated a total of 173 azulene-, tropolone- and azulenequinone-related compounds for their tumor-specificity and anti-inflammatory activity. In this study, we selected six compounds that showed tumor-specific cytotoxicity (referred to as group I compounds) and five compounds that inhibited nitric oxide production by activated macrophages (referred to as group II compounds) to investigate their possible hormetic and anti-radiation effects. We have established three oral normal cell type, human gingival fibroblast HGF-1, pulp cell HPC-1 and periodontal ligament fibroblast HPLF-1, from extracted teeth and periodontal tissue. These normal cells expressed p53 protein, regardless of the growth stage (either at growing or near confluent phase), more than oral squamous cell carcinoma cell line (HSC-2). Group I compounds slightly stimulated the growth of HPL-1 cells only at restricted durations and concentrations, but did not affect that of HGF-1 and HPC-1 cells, suggesting the minor hormetic effects displayed by these compounds. We established a new evaluation system for UV-induced cellular damage using an intact HSC-2 cell system in which sodium ascorbate (vitamin C) and gallic acid, but not N-acetyl-l-cysteine nor catalase, exerted protective effects. Three group I compounds and two group II compounds significantly protected the cells from UV-induced injury, suggesting their possible anti-UV effect.
我们之前研究了总共 173 种薁、色酮和薁醌类化合物，以研究它们的肿瘤特异性和抗炎活性。在这项研究中，我们选择了 6 种具有肿瘤特异性细胞毒性的化合物（称为 I 组化合物）和 5 种抑制激活的巨噬细胞产生一氧化氮的化合物（称为 II 组化合物），以研究它们可能的适应原和抗辐射作用。我们从提取的牙齿和牙周组织中建立了三种口腔正常细胞类型，人牙龈成纤维细胞 HGF-1、牙髓细胞 HPC-1 和牙周膜成纤维细胞 HPLF-1。这些正常细胞表达 p53 蛋白，无论生长阶段（生长或接近汇合阶段）如何，都比口腔鳞状癌细胞系（HSC-2）多。I 组化合物仅在有限的时间和浓度下轻微刺激 HPL-1 细胞的生长，但不影响 HGF-1 和 HPC-1 细胞的生长，表明这些化合物显示出轻微的适应原效应。我们使用完整的 HSC-2 细胞系统建立了一种新的评估紫外线诱导细胞损伤的系统，其中抗坏血酸钠（维生素 C）和没食子酸，但不是 N-乙酰-l-半胱氨酸或过氧化氢酶，发挥保护作用。三种 I 组化合物和两种 II 组化合物显著保护细胞免受 UV 诱导的损伤，表明它们可能具有抗 UV 作用。
