根癌土壤杆菌氯霉素抗性决定子的核苷酸序列分析及其基因产物的鉴定。
Nucleotide sequence analysis of a chloramphenicol-resistance determinant from Agrobacterium tumefaciens and identification of its gene product.
作者信息
Tennigkeit J, Matzura H
机构信息
Molekulare Genetik der Universität Heidelberg, F.R.G.
出版信息
Gene. 1991 Feb 1;98(1):113-6. doi: 10.1016/0378-1119(91)90112-o.
PMID:2013403
Abstract
The nucleotide sequence of a chloramphenicol-resistance (CmR) determinant from the Gram- soil bacterium Agrobacterium tumefaciens was determined, and its gene product was identified as Cm acetyltransferase (CAT). Comparison of the amino acid sequences of the A. tumefaciens CAT and various CAT proteins of Gram+ and Gram- origin shows no homology between this and the other enzymes.
摘要
测定了革兰氏阴性土壤细菌根癌农杆菌中氯霉素抗性（CmR）决定簇的核苷酸序列，并将其基因产物鉴定为Cm乙酰转移酶（CAT）。对根癌农杆菌CAT与革兰氏阳性和革兰氏阴性来源的各种CAT蛋白的氨基酸序列进行比较，结果表明该酶与其他酶之间不存在同源性。
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