Emig Dorothea, Cline Melissa S, Klein Karsten, Kunert Anne, Mutzel Petra, Lengauer Thomas, Albrecht Mario
Department of Computational Biology and Applied Algorithmics, Max Planck Institute for Informatics, Campus E1.4, 66123 Saarbrücken, Germany.
J Integr Bioinform. 2008 Aug 25;5(2):101. doi: 10.2390/biecoll-jib-2008-101.
Proteins and their interactions are essential for the functioning of all organisms and for understanding biological processes. Alternative splicing is an important molecular mechanism for increasing the protein diversity in eukaryotic cells. Splicing events that alter the protein structure and the domain composition can be responsible for the regulation of protein interactions and the functional diversity of different tissues. Discovering the occurrence of splicing events and studying protein isoforms have become feasible using Affymetrix Exon Arrays. Therefore, we have developed the versatile Cytoscape plugin DomainGraph that allows for the visual analysis of protein domain interaction networks and their integration with exon expression data. Protein domains affected by alternative splicing are highlighted and splicing patterns can be compared.
蛋白质及其相互作用对于所有生物体的功能以及理解生物过程至关重要。可变剪接是增加真核细胞中蛋白质多样性的重要分子机制。改变蛋白质结构和结构域组成的剪接事件可能负责调节蛋白质相互作用以及不同组织的功能多样性。使用Affymetrix外显子芯片发现剪接事件的发生并研究蛋白质异构体已变得可行。因此，我们开发了通用的Cytoscape插件DomainGraph，它允许对蛋白质结构域相互作用网络及其与外显子表达数据的整合进行可视化分析。受可变剪接影响的蛋白质结构域会被突出显示，并且可以比较剪接模式。
