Department of Biology, Gazi University, 06500, Teknikokullar, Ankara, Turkey.
J Microbiol Biotechnol. 2010 Jan;20(1):161-8. doi: 10.4014/jmb.0904.04019.
In this study, the metabolic activities of five strains of amount of Pediococcus spp. in terms of the quantities they produced of lactic acid, hydrogen peroxide, exopolysaccharides and proteolytic activity were determined. Lactic acid levels produced by these strains were found to be in the range of 2.5-5.6 mg/ml. All strains produced hydrogen peroxide. The P. pentosaceus Z13P strain produced the maximum amount (0.25 mg/ml) of proteolytic activity. Exopolysaccharide (EPS) production by the Pediococcus strains during growth in MRS medium was in the range 25-64 mg/l. The susceptibility of 10 different antibiotics against these strains was also tested. All strains were found to be resistant to amoxicillin, gentamicin, and vancomicin. Antimicrobial effects of the Pediococcus on pathogens were also determined by an agar diffusion method. All of the strains were able to inhibit L. monocytogenes. The tolerance of the strains to low pH, their resistance to bile salts of strains, and their abilities to autoaggregate and coaggregate with L. monocytogenes were also evaluated.
在这项研究中,测定了五株片球菌属(Pediococcus spp.)菌株的代谢活性,包括它们产生的乳酸、过氧化氢、胞外多糖和蛋白水解活性的量。这些菌株产生的乳酸水平在 2.5-5.6mg/ml 之间。所有菌株均产生过氧化氢。戊糖片球菌 Z13P 菌株产生的蛋白水解活性最高(0.25mg/ml)。在 MRS 培养基中生长时,片球菌属菌株的胞外多糖(EPS)产量在 25-64mg/l 之间。还测试了 10 种不同抗生素对这些菌株的敏感性。所有菌株均对阿莫西林、庆大霉素和万古霉素表现出耐药性。通过琼脂扩散法还确定了片球菌对病原体的抗菌作用。所有菌株均能抑制单核细胞增生李斯特菌。还评估了菌株对低 pH 的耐受性、对胆汁盐的抗性以及与单核细胞增生李斯特菌的自动聚集和共聚集能力。