Handa Shweta, Sharma Nivedita
Microbiology Research Laboratory, Department of Basic Sciences, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan 173230, HP, India.
J Genet Eng Biotechnol. 2016 Jun;14(1):91-97. doi: 10.1016/j.jgeb.2016.08.001. Epub 2016 Aug 29.
Present study was carried out to evaluate a new bacterial strain, F22 as probiotic strain. F22 was isolated from a traditional inoculum called 'Phab' which is used for the preparation of a traditional beverage chhang of Lahaul and Spiti of Himachal Pradesh. The isolate was identified by conventional and molecular techniques and tested for different probiotic properties. The 16S rRNA sequence of the isolate was registered in National Centre for Biotechnology Information (NCBI) under accession number . Further, F22 was evaluated for its probiotic potential viz., autoaggregation capacity, hydrophobicity, acidity tolerance, antibiotic susceptibility and cumulative probiotic potential and was found to possess good probiotic potential with a cumulative probiotic score of 91.7%. F22 has been proved to be highly effective, therefore can be recommended for the development of new pharmaceuticals and functional food preparations.
本研究旨在评估一种新的细菌菌株F22作为益生菌菌株的特性。F22是从一种名为“Phab”的传统接种物中分离出来的，该接种物用于制备喜马偕尔邦拉胡尔和斯皮提地区的传统饮料chhang。通过传统和分子技术对该分离株进行了鉴定，并对其不同的益生菌特性进行了测试。该分离株的16S rRNA序列已在国家生物技术信息中心（NCBI）注册，登录号为 。此外，对F22的益生菌潜力进行了评估，即自聚集能力、疏水性、耐酸性、抗生素敏感性和累积益生菌潜力，发现其具有良好的益生菌潜力，累积益生菌评分为91.7%。F22已被证明具有高效性，因此可推荐用于开发新的药物和功能性食品制剂。
