Josefowicz J, Hallett F R
Appl Opt. 1975 Mar 1;14(3):740-2. doi: 10.1364/AO.14.000740.
Homodyne light fluctuation spectroscopy is combined with an electrophoresis apparatus to provide a technique that rapidly measures electrophoretic mobilities of charged particles suspended in a liquid medium. Two parallel laser beams, polarized in the same direction, are focused into a scattering cell by a cylindrical lens. The laser beams interfere at the focal point of the lens to produce a fringe intensity profile. As scattering particles are driven through the fringes, the scattered light intensity is modulated. This modulation is detected by digital autocorrelation of photoelectron pulses from a photomultiplier. With this technique the electrophoretic mobility of 1-micro diam monodisperse polystyrene spheres was determined to be 4.6 +/- 0.2 (micro/sec)/(V/cm) in water.
零差光波动光谱法与电泳仪相结合，提供了一种能快速测量悬浮在液体介质中的带电粒子电泳迁移率的技术。两束沿相同方向偏振的平行激光束通过柱面透镜聚焦到一个散射池中。激光束在透镜的焦点处发生干涉，产生条纹强度分布。当散射粒子穿过条纹时，散射光强度会被调制。这种调制通过光电倍增管产生的光电子脉冲的数字自相关来检测。利用该技术，在水中测得1微米直径的单分散聚苯乙烯球体的电泳迁移率为4.6±0.2（微米/秒）/（伏/厘米）。
