Anttila Katja, Jäntti Maria, Mänttäri Satu
Department of Biology, University of Oulu, PO Box 3000, 90014 Oulu, Finland.
J Comp Physiol B. 2010 Jun;180(5):707-14. doi: 10.1007/s00360-010-0446-1. Epub 2010 Feb 5.
We examined the effect of exercise intensity and endurance training on plasma free fatty acid (FFA) kinetics and lipid metabolism in swimming muscles of reared sea trout. In both training groups [water current velocities 1 and 2 body lengths per second (bl s(-1))] the plasma level of FFAs decreased significantly (P < 0.001) compared to the control group. Similar significant (P < 0.01) post-exercise decrease was observed also in the lipase-esterase activity in the red muscle, but not in white. Moreover, in the group swimming with higher intensity a significantly higher (P < 0.05) lipase-esterase activity in the red muscle was found compared with the group on moderate exercise. As with cytochrome c oxidase activity, a significant elevation in the enzyme activity was also observed after training in the 1 bl s(-1) group in red and white muscle (P < 0.05 and P < 0.01, respectively). No changes were observed in beta hydroxyacyl CoA dehydrogenase activity. The lipid content was on average nine times higher in red compared to white muscle being 16.7, 21.1, and 24.9% in the red muscle of the control, 1 and 2 bl s(-1) groups, respectively, with a significant (P < 0.05) increase after training. We conclude that (1) unlike in mammals, plasma FFA kinetics and oxidation are not linearly related to exercise intensity in reared sea trout, (2) training enhances the capacity to uptake FFA from plasma, and (3) high intensity training shifts the proportion of energy derived from fat oxidation to carbohydrate-derived energy.
我们研究了运动强度和耐力训练对养殖海鳟游泳肌肉中血浆游离脂肪酸（FFA）动力学及脂质代谢的影响。与对照组相比，两个训练组（水流速度分别为每秒1和2个体长，即1 bl s⁻¹和2 bl s⁻¹）的血浆FFA水平均显著降低（P < 0.001）。红肌中的脂肪酶 - 酯酶活性在运动后也出现了类似的显著降低（P < 0.01），而白肌中则未出现。此外，高强度游泳组红肌中的脂肪酶 - 酯酶活性显著高于中等强度运动组（P < 0.05）。与细胞色素c氧化酶活性一样，1 bl s⁻¹训练组的红肌和白肌在训练后酶活性也显著升高（分别为P < 0.05和P < 0.01）。β - 羟酰基辅酶A脱氢酶活性未观察到变化。红肌中的脂质含量平均是白肌的9倍，对照组、1 bl s⁻¹组和2 bl s⁻¹组红肌中的脂质含量分别为16.7%、21.1%和24.9%，训练后显著增加（P < 0.05）。我们得出结论：（1）与哺乳动物不同，养殖海鳟血浆FFA动力学和氧化与运动强度并非线性相关；（2）训练可增强从血浆摄取FFA的能力；（3）高强度训练使脂肪氧化产生的能量比例向碳水化合物衍生的能量转移。
