Okada Yasunori
Department of Pathology, School of Medicine, Keio University, Tokyo, Japan.
Methods Mol Biol. 2010;622:211-9. doi: 10.1007/978-1-60327-299-5_12.
Immunohistochemistry is a useful and powerful method to determine the cells responsible for the production of MMPs and TIMPs and localize them to the tissue areas where they are functioning. This chapter describes the detailed methods of the immunohistochemistry applied to human pathological tissues using commercially available monoclonal antibodies against MMPs and TIMPs. Information about the monoclonal antibodies and solution of the problems with immunostaining is also provided.
免疫组织化学是一种有用且强大的方法，可用于确定产生基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制剂（TIMPs）的细胞，并将它们定位到其发挥作用的组织区域。本章描述了使用市售的针对MMPs和TIMPs的单克隆抗体，对人类病理组织进行免疫组织化学的详细方法。还提供了有关单克隆抗体的信息以及免疫染色问题的解决方案。
