Department of Bioengineering, University of California, Los Angeles, 5121 Engineering 5, Los Angeles, CA 90095, USA.
Macromol Biosci. 2010 May 14;10(5):496-502. doi: 10.1002/mabi.200900390.
The synthesis and self-assembly of DOPA containing diblock copolypeptides into spherical vesicles is described. DOPA residues are naturally abundant in mussel adhesive proteins and are responsible for extensive covalent crosslinking of these materials upon oxidation. We found that vesicles could be formed from copolypeptides containing different amounts of DOPA substituted into hydrophobic segments, up to 100% DOPA content. The DOPA containing vesicles were covalently crosslinked in water using an oxidizing agent, in a process similar to the crosslinking of mussel adhesive proteins, which gave vesicles with dramatically improved membrane stability against freeze-drying, organic solvent, osmotic stress and complex media. These materials showed greatly enhanced membrane stability compared to non-crosslinked vesicles and have the advantage that the biomimetic crosslinker DOPA can be incorporated directly into the polypeptide sequence during synthesis.
本文描述了含有 DOPA 的二嵌段多肽的合成和自组装成球形囊泡。DOPA 残基在贻贝类黏附蛋白中大量存在，这些材料在氧化过程中通过 DOPA 残基发生广泛的共价交联。我们发现，囊泡可以由含有不同含量 DOPA 取代疏水段的嵌段多肽形成，最高可达 100%的 DOPA 含量。使用氧化剂，DOPA 囊泡在水中进行共价交联，这一过程类似于贻贝类黏附蛋白的交联，使囊泡的膜稳定性得到显著提高，可耐受冻干、有机溶剂、渗透压和复杂介质。与非交联囊泡相比，这些材料具有显著增强的膜稳定性，并且具有的优势是，仿生交联剂 DOPA 可以在合成过程中直接整合到多肽序列中。
