Department of Pediatrics, University of Virginia Health System, Charlottesville, VA 22908, USA.
Pediatr Infect Dis J. 2010 Feb;29(2):102-4. doi: 10.1097/inf.0b013e3181b6e482.
Toys in pediatric office waiting rooms may be fomites for transmission of viruses.
Eighteen samples were taken from office objects on 3 occasions. Samples were tested for presence of picornavirus (either rhinovirus or enterovirus) on all 3 sample days; in addition, January samples were tested for respiratory syncytial virus and March samples were tested for influenza A and B. In addition, 15 samples were obtained from the sick waiting room before and after cleaning. Polymerase chain reaction was used to detect picornavirus, respiratory syncytial virus, and influenza A or B virus. Finally, 20 samples were obtained from the fingers of a researcher after handling different toys in the sick waiting room, and samples were then obtained from all the same toys; all samples were tested for picornavirus by polymerase chain reaction.
Viral RNA was detected on 11 of 52 (21%) of toys sampled. Ten of the positives were picornavirus; 1 was influenza B virus. Three (30%) of 10 toys from the new toy bag, 6 of 30 (20%) in the sick child waiting room, and 2 of 12 (17%) in the well child waiting room were positive. Six (40%) of 15 toys in the sick waiting room were positive for picornaviral RNA before cleaning; after cleaning, 4 (27%) of 15 were positive in spite of the fact that RNA was removed from 4 of 6 of the original positives. Three (15%) of 20 toys in the sick waiting room were positive for picornaviral RNA, but RNA was not transferred to the fingers of the investigator who handled these toys.
About 20% of the objects in a pediatric office may be contaminated with respiratory viral RNA, most commonly picornavirus RNA. Cleaning with a disinfectant cloth was only modestly effective in removing the viral RNA from the surfaces of toys, but transfer of picornaviral RNA from toys to fingers was inefficient.
儿科候诊室的玩具可能是病毒传播的媒介。
在 3 个时间点从办公物品上采集了 18 个样本。所有 3 个采样日均对样本进行了检测，以确定是否存在小核糖核酸病毒（鼻病毒或肠道病毒）；此外，1 月份的样本检测了呼吸道合胞病毒，3 月份的样本检测了流感 A 和 B。此外，在清洁前后，从候诊室的病人处采集了 15 个样本。聚合酶链反应用于检测小核糖核酸病毒、呼吸道合胞病毒和流感 A 或 B 病毒。最后，研究人员在病人候诊室处理不同玩具后，从手指上采集了 20 个样本，然后从所有相同的玩具上采集了样本；所有样本均通过聚合酶链反应检测小核糖核酸病毒。
在所采集的 52 个玩具样本中，有 11 个（21%）检测到病毒 RNA。10 个阳性结果是小核糖核酸病毒；1 个是乙型流感病毒。新玩具袋中有 3 个（30%）、病人候诊室中有 30 个中的 6 个（20%）、健康儿童候诊室中有 12 个中的 2 个（17%）玩具为阳性。清洁前，病人候诊室中 15 个玩具中有 6 个（40%）检测到小核糖核酸病毒 RNA 阳性；尽管从 6 个原始阳性样本中清除了 RNA，但清洁后仍有 4 个（27%）阳性。病人候诊室中 20 个玩具中有 3 个（15%）检测到小核糖核酸病毒 RNA，但这些玩具未将 RNA 传递给处理玩具的研究人员的手指。
儿科诊室中约 20%的物品可能受到呼吸道病毒 RNA 的污染，最常见的是小核糖核酸病毒 RNA。用消毒布清洁只能适度地从玩具表面清除病毒 RNA，但小核糖核酸病毒 RNA 从玩具转移到手指的效率较低。
