Imano Motohiro, Satou Takao, Itoh Tatsuki, Takeyama Yoshifumi, Yasuda Atsushi, Peng Ying-Feng, Shinkai Masayuki, Haji Seiji, Yasuda Chikao, Nakai Takuya, Yasuda Takushi, Imamoto Haruhiko, Okuno Kiyotaka, Shiozaki Hitoshi, Ohyanagi Harumasa
Department of Surgery, Kinki University School of Medicine, Osaka, Japan.
Am Surg. 2010 Jan;76(1):91-5.
Mucin glycoproteins from the gallbladder epithelium are thought to contribute to the matrix or nucleus of gallstones and other biomineralization systems. The involved acidic glycoproteins have been reported in bile and gallstones. In addition, osteopontin (Opn) is a noncollagenous acidic bone matrix glycoprotein that possesses calcium-binding properties. To investigate the role of Opn in pigment gallstone formation, the involvement of Opn in pigment gallstone formation was studied immunohistochemically in the gallbladder wall and in the stones. Staining for Opn was strongly positive in the epithelium of stone-laden gallbladders and in their stones. The stone-laden gallbladders were infiltrated by macrophages, which intensely stained for Opn. Sections of the pigment stones, under low magnification, showed a lamellar pattern of Opn immunolabeling and showed a reticular pattern under high magnification. Our results indicate that Opn, an acidic glycoprotein from the gallbladder epithelium, seems to be involved in lithiasis. Opn from macrophages and/or the epithelium seems to help form the matrix protein.
胆囊上皮的黏蛋白糖蛋白被认为对胆结石及其他生物矿化系统的基质或核心有贡献。胆汁和胆结石中已报道了相关的酸性糖蛋白。此外，骨桥蛋白（Opn）是一种具有钙结合特性的非胶原蛋白酸性骨基质糖蛋白。为研究Opn在色素性胆结石形成中的作用，采用免疫组织化学方法研究了Opn在胆囊壁和结石中色素性胆结石形成中的作用。在充满结石的胆囊上皮及其结石中，Opn染色呈强阳性。充满结石的胆囊中有巨噬细胞浸润，巨噬细胞对Opn染色强烈。色素结石切片在低倍镜下显示Opn免疫标记呈层状模式，在高倍镜下呈网状模式。我们的结果表明，来自胆囊上皮的酸性糖蛋白Opn似乎参与了结石形成。来自巨噬细胞和/或上皮的Opn似乎有助于形成基质蛋白。
