Zhang Ming-Kui, Liu Zhao-Yun
Institute of Soil and Water Resource and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, China.
Ying Yong Sheng Tai Xue Bao. 2009 Nov;20(11):2790-8.
An incubation test was conducted to study the effects of antibiotics oxytetracycline on the microbial number and activity in three soils varied with fertility level under the conditions with or without pig manure application. The soils were injected with six concentrations (0, 0.1, 1, 10, 100, and 1000 mg x kg(-1)) oxytetracycline, and incubated for 30 d at 25 degrees C. Soil samples were collected on the 1st, 4th, and 30th day of incubation to measure the bacterial number, respiratory activity, and enzyme activity. Under no pig manure application, oxytetracycline had little effects on the soil bacterial number and activity. The EC10 values of oxytetracycline based on a 10% decrement of bacterial number, respiratory activity, enzyme activity, and NO3(-)-N concentration in treatment without pig manure were 36-1000 mg x kg(-1), 20-1000 mg x kg(-1), and 4-1000 mg x kg(-1) for S1, S2 and S3, while those in treatment with pig manure application were 2-656 mg x kg(-1), 2-81 mg x kg(-1), and 1-42 mg x kg(-1) for S1, S2 and S3, respectively. Soil fertility level had obvious effects on the dose-response relations between oxytetracycline and soil microbial number and activity. The effects of oxytetracycline on soil microbial number and activity increased with increasing soil fertility level, and the effects on microbial number and respiratory activity were higher than those on enzyme activity and NO3(-)-N concentration. The effects of oxytetracycline on soil microbial number and activity were time-depending, being the greatest on the 4th day of incubation. Overall speaking, oxytetracycline could exert temporary inhibition on soil microbes.
进行了一项培养试验，以研究在施用或不施用猪粪的条件下，抗生素土霉素对三种肥力水平不同的土壤中微生物数量和活性的影响。向土壤中注入六种浓度（0、0.1、1、10、100和1000 mg·kg⁻¹）的土霉素，并在25℃下培养30天。在培养的第1天、第4天和第30天采集土壤样品，以测量细菌数量、呼吸活性和酶活性。在不施用猪粪的情况下，土霉素对土壤细菌数量和活性影响较小。在不施用猪粪的处理中，基于细菌数量、呼吸活性、酶活性和NO₃⁻-N浓度降低10%的土霉素EC₁₀值，S1、S2和S3分别为36 - 1000 mg·kg⁻¹、20 - 1000 mg·kg⁻¹和4 - 1000 mg·kg⁻¹；而在施用猪粪的处理中，S1、S2和S3的EC₁₀值分别为2 - 656 mg·kg⁻¹、2 - 81 mg·kg⁻¹和1 - 42 mg·kg⁻¹。土壤肥力水平对土霉素与土壤微生物数量和活性之间的剂量反应关系有明显影响。土霉素对土壤微生物数量和活性的影响随土壤肥力水平的提高而增加，且对微生物数量和呼吸活性的影响高于对酶活性和NO₃⁻-N浓度的影响。土霉素对土壤微生物数量和活性的影响具有时间依赖性，在培养第4天时影响最大。总体而言，土霉素可对土壤微生物产生暂时抑制作用。
