Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
Anal Chem. 2010 Mar 1;82(5):2042-7. doi: 10.1021/ac902791q.
We demonstrate a highly sensitive nano aptasensor for anthrax toxin through the detection of its polypeptide entity, protective antigen (PA toxin) using a PA toxin ssDNA aptamer functionalized single-walled carbon nanotubes (SWNTs) device. The aptamer was developed in-house by capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) and had a dissociation constant (K(d)) of 112 nM. The aptasensor displayed a wide dynamic range spanning up to 800 nM with a detection limit of 1 nM. The sensitivity was 0.11 per nM, and it was reusable six times. The aptasensor was also highly selective for PA toxin with no interference from human and bovine serum albumin, demonstrating it as a potential tool for rapid and point-of-care diagnosis for anthrax.
我们通过使用 PA 毒素 ssDNA 适体功能化单壁碳纳米管 (SWNTs) 器件来检测其多肽实体保护性抗原 (PA 毒素),展示了一种针对炭疽毒素的高灵敏度纳米适体传感器。该适体是通过毛细管电泳系统进化的配体指数富集 (CE-SELEX) 内部开发的,解离常数 (Kd) 为 112 nM。该适体传感器的动态范围很宽,可达 800 nM,检测限为 1 nM。其灵敏度为 0.11 皮摩尔/纳米,可重复使用六次。该适体传感器对 PA 毒素具有高度选择性,不受人血清白蛋白和牛血清白蛋白的干扰,表明它是一种用于炭疽快速即时诊断的潜在工具。