原位杂交的前景。
In situ hybridisation in perspective.
作者信息
Warford A, Lauder I
机构信息
Department of Pathology, Leicester Royal Infirmary.
出版信息
J Clin Pathol. 1991 Mar;44(3):177-81. doi: 10.1136/jcp.44.3.177.
Abstract
In the introduction to this review two questions were posed: is the technology associated with ISH ready for general use, and will the method become an important investigative tool? With the exception of the demonstration of some single and low copy sequences, non-radioactive ISH is now sufficiently developed and simplified to make it a routine technique. It is also clear that ISH will continue to have an important research role. In diagnostic pathology the technique is already providing valuable information and the present decade should see the development of many more diagnostic applications.
摘要
在本综述的引言部分提出了两个问题
与原位杂交（ISH）相关的技术是否已准备好普遍应用，以及该方法是否会成为一种重要的研究工具？除了一些单拷贝和低拷贝序列的展示外，非放射性原位杂交现已得到充分发展和简化，使其成为一种常规技术。同样明显的是，原位杂交将继续发挥重要的研究作用。在诊断病理学中，该技术已经在提供有价值的信息，并且在当前十年应该会出现更多诊断应用的发展。
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