Davis M, Malcolm S, Rabbitts T H
Nature. 1984;308(5956):286-8. doi: 10.1038/308286a0.
In Burkitt lymphoma cells reciprocal chromosomal translocations are observed between the long arm of chromosome 8 (8q24) and either the long arm of chromosome 14 (14q32), the short arm of chromosome 2 (2p12) or the long arm of chromosome 22 (22q11). Gene mapping studies have shown that c-myc, the cellular homologue of the viral myc oncogene, is localized at 8q24 (ref 3-5) and that the three immunoglobulin gene loci map at the breakpoints involved in the translocation: immunoglobulin heavy-chain genes are located at 14q32 (refs 6, 7), kappa light chains at 2p12 (ref. 8) and delta light chains at, or close to, 22q11 (ref 9, 10). This correlation suggests an association of immunoglobulin gene rearrangements with the occurrence of these specific translocations. By using in situ hybridization we have examined the translocation point with respect to c-myc in two cell lines containing 2;8 translocation, and report here that the c-myc gene remains on the chromosome 8 involved in the reciprocal exchange with chromosome 2. We have also confirmed that the c-myc gene moves from the translocated chromosome 8 in a cell line having a 8;14 translocation. These results show that chromosomal breakage can occur on either side of the c-myc gene in Burkitt lymphoma cells.
在伯基特淋巴瘤细胞中，可观察到8号染色体长臂（8q24）与14号染色体长臂（14q32）、2号染色体短臂（2p12）或22号染色体长臂（22q11）之间发生相互染色体易位。基因定位研究表明，病毒myc癌基因的细胞同源物c-myc定位于8q24（参考文献3 - 5），并且三个免疫球蛋白基因位点定位于易位所涉及的断点处：免疫球蛋白重链基因位于14q32（参考文献6, 7），κ轻链位于2p12（参考文献8），δ轻链位于22q11或其附近（参考文献9, 10）。这种相关性表明免疫球蛋白基因重排与这些特定易位的发生有关。通过原位杂交，我们在两个含有2;8易位的细胞系中研究了相对于c-myc的易位点，并在此报告c-myc基因仍位于与2号染色体发生相互交换的8号染色体上。我们还证实，在一个具有8;14易位的细胞系中，c-myc基因从易位的8号染色体上移动。这些结果表明，在伯基特淋巴瘤细胞中，c-myc基因两侧均可发生染色体断裂。
