Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
J Neurosurg. 2010 Oct;113(4):820-5. doi: 10.3171/2010.1.JNS09637.
Spontaneous intracerebral hemorrhage (ICH) is among the most intractable forms of stroke. Angiogenesis, an orchestrated balance between proangiogenic and antiangiogenic factors, is a fundamental process to brain development and repair by new blood vessel formation from preexisting ones and can be induced by ICH. Thrombospondin (TSP)–1 and TSP-2 are naturally occurring antiangiogenic factors. The aim of this study was to observe their expression in rat brains with ICH.
Intracerebral hemorrhage was induced in adult male Sprague-Dawley rats by stereotactic injection of collagenase VII or autologous blood into the right globus pallidus. The expression of TSP-1 and -2 was evaluated by immunohistochemistry and quantitative real-time reverse transcription–polymerase chain reaction analysis.
After the induction of ICH, some TSP1- or TSP2-immunoreactive microvessels resided around the hematoma for ~ 7 days and extended into a clot thereafter. Cerebral endothelial cells expressed the TSPs. The expression of TSP-1 and TSP-2 mRNA peaked at 4 and 14 days after collagenase-induced ICH, respectively.
Findings in this study suggest that ICH can alter the expression of TSP-1 and TSP-2, which may be involved in modulating angiogenesis in brains following ICH.
自发性脑出血（ICH）是最顽固的中风形式之一。血管生成是一种协调的促血管生成和抗血管生成因子之间的平衡，是大脑发育和修复的基本过程，通过从预先存在的血管形成新的血管来实现，并且可以通过 ICH 诱导。血栓素（TSP）-1 和 TSP-2 是天然存在的抗血管生成因子。本研究旨在观察它们在脑出血大鼠大脑中的表达。
通过立体定向向右侧苍白球内注射胶原酶 VII 或自体血，在成年雄性 Sprague-Dawley 大鼠中诱导脑出血。通过免疫组织化学和实时定量逆转录-聚合酶链反应分析评估 TSP-1 和 -2 的表达。
ICH 诱导后，一些 TSP1 或 TSP2 免疫反应性微血管在血肿周围存在约 7 天，并在此后延伸至凝块中。脑内皮细胞表达 TSP。TSP-1 和 TSP-2 mRNA 的表达分别在胶原酶诱导的 ICH 后 4 天和 14 天达到峰值。
本研究的结果表明，ICH 可以改变 TSP-1 和 TSP-2 的表达，这可能参与调节 ICH 后大脑中的血管生成。
