Department of Biosciences, Graduate School of Science, Kitasato University, Sagamihara, Kanagawa, Japan.
Immunopharmacol Immunotoxicol. 2010 Dec;32(4):593-9. doi: 10.3109/08923971003604786. Epub 2010 Feb 8.
We investigated the inhibitory effects of fermented grape marc (FGM), lyophilized fine powder of skin, and seeds of Vitis vinifera Koshu grape prepared by fermentation with Lactobacillus plantarum, on type-I allergic responses in mice. Repeated oral administration of FGM, but not non-fermented grape marc (GM), to BALB/c mice primed with ovalbumin (OVA) resulted in a significant reduction of serum IgE levels, compared with those of immunized controls. After OVA challenge, increased numbers of eosinophils in bronchial alveolar lavage fluids (BALF) significantly decreased by treatment with FGM but not with GM. For passive cutaneous anaphylaxis (PCA) reaction, BALB/c mice received intradermal sensitization with anti-OVA IgE serum and were challenged intravenously with OVA containing Evans blue at 24 h after IgE sensitization. Oral administration of FGM at 30 min before OVA challenge significantly suppressed the PCA reaction. On the other hand, Lactobacillus alone and non-fermented GM did not show any suppressive effects. Interestingly, FGM samples prepared from grapes for red wine, such as Negroamaro (rich in resveratrol) or Tannat (rich in oligomeric procyanidin), did not suppress the reaction. These results indicate that oral administration of FGM, prepared from Koshu grape for white wine but not from grapes for red wine, could suppress both phases of type-I allergic responses. A fraction extractable with acetone was responsible for the suppressive effects of FGM.
我们研究了发酵葡萄渣（FGM）、冻干的葡萄皮和种子粉末，以及用植物乳杆菌发酵的甲州葡萄的细粉，对小鼠 I 型过敏反应的抑制作用。与免疫对照组相比，反复口服 FGM（而非未发酵的葡萄渣（GM））处理的卵清蛋白（OVA）预致敏 BALB/c 小鼠，其血清 IgE 水平显著降低。在用 FGM 处理后，OVA 攻击后支气管肺泡灌洗液（BALF）中嗜酸性粒细胞数量显著减少，但 GM 处理则没有。对于被动皮肤过敏（PCA）反应，BALB/c 小鼠接受抗 OVA IgE 血清的皮内致敏，并在 IgE 致敏后 24 小时内静脉内用含 Evans 蓝的 OVA 进行挑战。在 OVA 攻击前 30 分钟口服 FGM 可显著抑制 PCA 反应。另一方面，单独的乳杆菌和未发酵的 GM 均没有显示出任何抑制作用。有趣的是，FGM 样品来自红葡萄酒用的葡萄，如 Negroamaro（富含白藜芦醇）或 Tannat（富含低聚原花青素），则不能抑制反应。这些结果表明，口服 FGM（来自白葡萄酒用的甲州葡萄，而不是红葡萄酒用的葡萄）可以抑制 I 型过敏反应的两个阶段。用丙酮提取的馏分负责 FGM 的抑制作用。
