Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, Gwang-ju, 300, Yongbong-Dong, South Korea.
Immunol Invest. 2010 Jan;39(2):171-85. doi: 10.3109/08820130903513449.
The current study investigated whether the phytoglycoprotein (75 kDa) isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) has anti-allergic inflammatory potential. Bisphenol A (BPA) is well known to promote development of allergy-related immune dysfunction. This experiment evaluated the release of histamine, increased intracellular Ca(2+) level, and the activities of protein kinase C alpha (PKC alpha), transcription factors NFkappaB and cytokines (IL-4, and IFN-gamma) in BPA-treated RBL-2H3 cells. The results in this study showed that the phytoglycoprotein (75 kDa) inhibits the intracellular Ca(2+) level, translocation of PKC alpha from cytosol to membrane and the translocation of NF-kappaB in cells. We also found that the phytoglycoprotein (75 kDa) inhibits the release of histamine, and the expressions of cytokines (IL-4 and IFN-gamma) in the presence of BPA in RBL-2H3 cells. The results obtained from these experiments indicated that the phytoglycoprotein (75 kDa) inhibits release of histamine and expressions of IL-4, and IFN-gamma via down regulations of Ca(2+)/PKCalpha and NF-kappaB on the stage of degranulation induced by BPA. From point of view, we speculated that the phytoglycoprotein (75 kDa) is one of natural compounds that can block allergic-inflammation caused by BPA.
本研究探讨了从枳椇（Cudrania tricuspidata Bureau）中分离得到的植物糖蛋白（75 kDa）是否具有抗过敏性炎症的潜力。双酚 A（BPA）是众所周知的促进与过敏相关的免疫功能障碍发展的物质。本实验评估了 BPA 处理的 RBL-2H3 细胞中组胺的释放、细胞内 Ca(2+)水平的增加、蛋白激酶 Cα（PKCα）、转录因子 NFkappaB 和细胞因子（IL-4 和 IFN-γ）的活性。本研究结果表明，植物糖蛋白（75 kDa）抑制细胞内 Ca(2+)水平、PKCα从细胞质向膜的易位以及 NF-kappaB 在细胞内的易位。我们还发现，植物糖蛋白（75 kDa）抑制组胺的释放以及 BPA 存在时 RBL-2H3 细胞中细胞因子（IL-4 和 IFN-γ）的表达。这些实验结果表明，植物糖蛋白（75 kDa）通过下调 Ca(2+)/PKCalpha 和 NF-kappaB，抑制由 BPA 诱导的脱颗粒阶段的组胺释放和 IL-4、IFN-γ的表达。从这一点来看，我们推测植物糖蛋白（75 kDa）是一种可以阻断 BPA 引起的过敏炎症的天然化合物之一。
