植物糖蛋白（75 kDa）抑制 DEHP 刺激的人肥大细胞中白细胞介素-1β的表达。

Phytoglycoprotein (75 kDa) inhibits expression of interleukin-1beta stimulated by DEHP in human mast cells.

机构信息

Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, Yongbong-Dong, Gwang-ju, South Korea.

出版信息

Cell Biochem Funct. 2010 Jul;28(5):352-9. doi: 10.1002/cbf.1648.

DOI:10.1002/cbf.1648

PMID:20586103

Abstract

The purpose of this study is to investigate the inhibitory effect of a glycoprotein (CTB glycoprotein, 75 kDa) isolated from Cudrania tricuspidata Bureau (CTB) on the di-(2-ethylhexyl)phthalate (DEHP) induced expression of allergic-inflammation-related mediators in human mast cells. The changes on the levels of intracellular reactive oxygen species (ROS), mitogen-activated protein kinase (MAPK), transcription factor [nuclear factor (NF)-kappaB], and allergic inflammatory mediators [cyclooxygenase (COX)-2 and interleukin (IL)-1beta] were evaluated using Western blot and RT-PCR. Our results showed that the CTB glycoprotein in the presence of DEHP inhibits the production of intracellular ROS, the phosphorylation of ERK1/2, and p38 MAPK in HMC-1 cells. In addition, the CTB glycoprotein has suppressive effects on the transcriptional activation of NF-kappaB, and on the expression levels of COX-2 and IL-1beta in DEHP-treated HMC-1 cells. In conclusion, the CTB glycoprotein has a strong anti-inflammatory effect on the activities of allergic inflammatory mediators indirectly caused by DEHP in HMC-1 cells.

摘要

本研究旨在探讨从枳椇（CTB）中分离得到的糖蛋白（CTB 糖蛋白，75kDa）对邻苯二甲酸二（2-乙基己基）酯（DEHP）诱导的人肥大细胞中过敏炎症相关介质表达的抑制作用。采用 Western blot 和 RT-PCR 方法评价细胞内活性氧（ROS）、丝裂原激活蛋白激酶（MAPK）、转录因子[核因子（NF）-κB]和过敏炎症介质[环氧化酶（COX）-2 和白细胞介素（IL）-1β]水平的变化。结果表明，CTB 糖蛋白在 DEHP 存在的情况下可抑制 HMC-1 细胞内 ROS 的产生、ERK1/2 和 p38 MAPK 的磷酸化。此外，CTB 糖蛋白对 NF-κB 的转录激活以及 DEHP 处理的 HMC-1 细胞中 COX-2 和 IL-1β的表达水平具有抑制作用。综上所述，CTB 糖蛋白对 HMC-1 细胞中由 DEHP 间接引起的过敏炎症介质的活性具有较强的抗炎作用。