Vinayak V K, Dutt P, Puri M
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
J Immunol Methods. 1991 Mar 21;137(2):245-51. doi: 10.1016/0022-1759(91)90030-j.
A dot-ELISA technique for the detection of G. lamblia specific antigen in stool eluates of clinical cases of giardiasis was developed and evaluated employing monospecific antibodies to a G. lamblia specific coproantigen with a molecular mass of 66 kDa. The assay detected 22 (91.7%) of the 24 microscopically confirmed cases of giardiasis while none of the stool eluates from 20 patients with gastrointestinal parasites other than G. lamblia and 20 apparently healthy subjects had any detectable levels of G. lamblia-specific coproantigen. Of the 25 stool eluates from clinically suspected cases of giardiasis, 13 (52%) were found to contain G. lamblia-specific coproantigen. A 3-month-follow up of five of such cases where stool eluates has antigen detected by dot-ELISA assay, revealed the presence of G. lamblia cysts on repeated stool examinations. All the clinically suspected cases with detectable levels of G. lamblia-specific coproantigen by dot-ELISA were relieved of their clinical symptoms following metronidazole therapy. Single stool eluate examination by dot-ELISA was found to be sufficient to confirm the diagnosis. The dot-ELISA is an easy, rapid, sensitive and specific procedure for confirming the diagnosis of suspected cases of giardiasis. It should be a valuable diagnostic aid under field conditions as well as in the laboratory.
利用针对贾第虫属一种分子量为66 kDa的特异性粪抗原的单特异性抗体，开发并评估了一种用于检测贾第虫病临床病例粪便洗脱液中贾第虫属特异性抗原的斑点酶联免疫吸附测定（dot-ELISA）技术。该检测方法在24例经显微镜确诊的贾第虫病病例中检测出22例（91.7%），而在20例感染除贾第虫属之外的肠道寄生虫的患者以及20例明显健康的受试者的粪便洗脱液中，均未检测到任何可检测水平的贾第虫属特异性粪抗原。在25份临床疑似贾第虫病病例的粪便洗脱液中，有13份（52%）被发现含有贾第虫属特异性粪抗原。对其中5例粪便洗脱液经斑点酶联免疫吸附测定检测出抗原的病例进行了为期3个月的随访，结果显示在重复的粪便检查中发现了贾第虫囊肿。所有经斑点酶联免疫吸附测定检测出可检测水平的贾第虫属特异性粪抗原的临床疑似病例，在接受甲硝唑治疗后临床症状均得到缓解。发现通过斑点酶联免疫吸附测定对单次粪便洗脱液进行检查足以确诊。斑点酶联免疫吸附测定是一种用于确诊疑似贾第虫病病例的简便、快速、灵敏且特异的方法。在现场条件以及实验室中，它都应是一种有价值的诊断辅助手段。
