Sugi T, Makino T, Toyoshima K, Sakai A, Maruyama T, Nozawa S, Iizuka R
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo.
Nihon Sanka Fujinka Gakkai Zasshi. 1991 Feb;43(2):173-8.
The mechanism of the beneficial effect of immunotherapy for human reproductive wastage remains to be elucidated. The induction of blocking antibodies such as anti-HLA class II antibodies and anti-idiotypic antibodies was investigated as the mechanism of specific immunosuppression in pregnancy. We reported that changes in a mixed lymphocyte culture (MLC), T-cell subsets and generation of anti-idiotypic antibodies after immunotherapy compared to before. MLC was significantly (p less than 0.001) inhibited after the immunization. The mean inhibition rate was 50.5%, suggesting that MLR blocking antibodies were induced by immunotherapy. We demonstrated that this blocking effect was specific to the husband, because the inhibition of one-way MLC reactions which were set up with responding maternal lymphocytes and MMC-treated stimulating lymphocytes of third parties were significantly low (p less than 0.05) compared to stimulation with paternal lymphocytes. The binding of autoantibodies to alloactivated maternal lymphoblasts against the paternal lymphocytes was detected in post-immunization cases in two-color flow-cytometric experiments. This strongly suggests that anti-idiotypic antibodies were induced by the immunotherapy. The percentage of cytotoxic T cells was significantly decreased (p less than 0.05) and the percentage of suppressor T cells was significantly increased (p less than 0.01) after the immunotherapy, suggesting that a cell-mediated immune response was induced by the immunotherapy.
免疫疗法对人类生殖障碍有益作用的机制仍有待阐明。作为妊娠特异性免疫抑制的机制，对诸如抗II类组织相容性复合体（HLA）抗体和抗独特型抗体等封闭抗体的诱导进行了研究。我们报告了免疫疗法前后混合淋巴细胞培养（MLC）、T细胞亚群以及抗独特型抗体生成的变化。免疫后MLC受到显著抑制（p<0.001）。平均抑制率为50.5%，表明免疫疗法诱导了混合淋巴细胞反应（MLR）封闭抗体。我们证明这种封闭效应是针对丈夫的，因为与用父亲淋巴细胞刺激相比，用反应性母体淋巴细胞和经丝裂霉素C（MMC）处理的第三方刺激淋巴细胞建立的单向MLC反应的抑制作用显著较低（p<0.05）。在双色流式细胞术实验中，在免疫后的病例中检测到自身抗体与针对父亲淋巴细胞的同种异体活化母体淋巴母细胞的结合。这有力地表明免疫疗法诱导了抗独特型抗体。免疫疗法后，细胞毒性T细胞的百分比显著降低（p<0.05），抑制性T细胞的百分比显著增加（p<0.01），表明免疫疗法诱导了细胞介导的免疫反应。
