Yamada K
Department of Obstetrics and Gynecology, Niigata University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1993 Jun;45(6):527-32.
The efficacy of immunotherapy with paternal lymphocytes has been reported as a treatment for unexplained habitual aborters. The underlying mechanisms, however, have not yet been fully elucidated. We have previously reported the appearance of humoral blocking factors which inhibited the response of maternal lymphocytes to paternal cells. Although studies concerning changes in cellular immunity in vaccinated patients have been reported, further analyses are required to elucidate the underlying mechanisms. In this context, we have investigated the changes in cytotoxic T lymphocyte precursor (CTL-p) frequency in 10 patients who were unexplained recurrent aborters before and after vaccination with the husband's lymphocytes using a sensitive limiting dilution culture system. The results obtained were as follows; 1. The reciprocal number for the frequency of CTL-p in patients reacting to their husband's lymphocytes before immunotherapy was 93,620.0 +/- 61,893.3 and that after the immunotherapy was 33,860.0 +/- 33,578.8. The frequency, thus, significantly increased after the immunotherapy (p < 0.01). 2. The frequency of CTL-p reacting to an unrelated third party's lymphocytes showed no significant change before and after the immunotherapy even though the HLA antigens in the husband and the third party were the same. 3. The sera, which were collected after the immunotherapy, were observed to undergo a decrease in the frequency of CTL-p.
据报道，用父亲的淋巴细胞进行免疫治疗对不明原因习惯性流产者有疗效。然而，其潜在机制尚未完全阐明。我们之前曾报道过体液阻断因子的出现，这些因子会抑制母体淋巴细胞对父方细胞的反应。尽管已有关于接种疫苗患者细胞免疫变化的研究报道，但仍需进一步分析以阐明潜在机制。在此背景下，我们使用敏感的极限稀释培养系统，研究了10名不明原因复发性流产患者在接种丈夫的淋巴细胞前后细胞毒性T淋巴细胞前体（CTL-p）频率的变化。结果如下：1. 免疫治疗前对丈夫淋巴细胞有反应的患者中CTL-p频率的倒数为93,620.0 +/- 61,893.3，免疫治疗后为33,860.0 +/- 33,578.8。因此，免疫治疗后频率显著增加（p < 0.01）。2. 尽管丈夫和第三方的HLA抗原相同，但免疫治疗前后对无关第三方淋巴细胞有反应的CTL-p频率无显著变化。3. 观察到免疫治疗后采集的血清中CTL-p频率降低。
