Iijima N, Sekiya S, Tokita H, Takamizawa H
Department of Obstetrics and Gynecology, Chiba University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1991 Feb;43(2):191-6.
By means of 3 different kinds of in vitro chemosensitivity testings--(1) a nuclear damage assay developed by us, (2) MTT assay, and (3) colony formation inhibition assay--we examined the sensitivity of 8 kinds of human ovarian cancer cell lines to various anticancer drugs. The sensitivity of in vivo xenografts of the cell lines in nude mice to anticancer drugs was also examined by inhibition of the tumor growth. The in vitro--in vivo correlation of sensitivity was studied in respect to both sensitivity and specificity rates. 1. Different active anticancer drugs were screened among the 3 in vitro chemosensitivity testings in the same human ovarian cancer cell line. 2. The in vitro--in vivo correlation of the nuclear damage assay (sensitivity 50%, specificity 94%) was the highest among the 3 testings. The nuclear damage assay which we developed therefore seemed to be the most useful assay method for clinical use.
通过3种不同的体外化学敏感性测试方法——（1）我们开发的核损伤检测法，（2）MTT检测法，以及（3）集落形成抑制检测法——我们检测了8种人卵巢癌细胞系对各种抗癌药物的敏感性。还通过抑制肿瘤生长检测了裸鼠体内这些细胞系异种移植物对抗癌药物的敏感性。从敏感性和特异性率两方面研究了体外-体内敏感性的相关性。1. 在同一人卵巢癌细胞系的3种体外化学敏感性测试中筛选出了不同的活性抗癌药物。2. 核损伤检测法（敏感性50%，特异性94%）的体外-体内相关性在3种测试中最高。因此，我们开发的核损伤检测法似乎是临床上最有用的检测方法。
