Cui Jiang-Xia, Pei Min-Fei, Zhang Guang-Sen, Xu Min
Department of Hematology, The Sencond Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Feb;18(1):111-5.
The objective of this study was to detect the expression frequency of HLA-DR15 in patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS), to investigate the relation of expression frequency with diseases and to analyze the relationship between immunoglobulin, T lymphocyte subsets and HLA-DR15. HLA-DR15 expression was detected by PCR-SSP; immunoglobulin was detected by immune turbidimetry; T cell subsets were detected by flow cytometry. The results showed that the expression rates of HLA-DR15 in AA and MDS as well as normal control groups were 78.6%, 63.2% and 24.6% respectively. The difference between AA, MDS and the normal control groups was statistically significant (p < 0.01). OR (odds ratios) values of AA and MDS groups were 11.262, 4.710 respectively. Compared with normal control group, expression rate of HLA-DR15 in hematologic malignancy group was not significantly different. The immunoglobulin level and abnormal T cell subsets in AA and MDS groups were statistically different between HLA-DR15 positive and negative groups (p > 0.05). It is concluded that the frequency of HLA-DR15 antigen in AA and MDS patients is significantly higher than that in normal control and hematologic malignancy group. OR value>1 showed a positive correlation between the diseases and HLA-DR15. HLA-DR15 is a susceptible gene in AA and MDS. The abnormalities of immunoglobulin level and ratios of T cell subsets in AA and MDS are common, but are not associated significantly with the expression of HLA-DR15.
本研究旨在检测再生障碍性贫血（AA）和骨髓增生异常综合征（MDS）患者中HLA - DR15的表达频率，探讨其表达频率与疾病的关系，并分析免疫球蛋白、T淋巴细胞亚群与HLA - DR15之间的关系。采用聚合酶链反应 - 序列特异性引物法（PCR - SSP）检测HLA - DR15表达；采用免疫比浊法检测免疫球蛋白；采用流式细胞术检测T细胞亚群。结果显示，AA组、MDS组及正常对照组中HLA - DR15的表达率分别为78.6%、63.2%和24.6%。AA组、MDS组与正常对照组之间的差异具有统计学意义（p < 0.01）。AA组和MDS组的优势比（OR）值分别为11.262、4.710。与正常对照组相比，血液系统恶性肿瘤组中HLA - DR15的表达率无显著差异。AA组和MDS组中，HLA - DR15阳性组与阴性组的免疫球蛋白水平及异常T细胞亚群存在统计学差异（p > 0.05）。结论：AA和MDS患者中HLA - DR15抗原频率显著高于正常对照组和血液系统恶性肿瘤组。OR值>1表明疾病与HLA - DR15呈正相关。HLA - DR15是AA和MDS的易感基因。AA和MDS中免疫球蛋白水平及T细胞亚群比例异常常见，但与HLA - DR15的表达无显著相关性。
