Lu Ying, Wang Jin-fen
Department of Ophthalmology, Beijing Electric Power Hospital, Beijing 100073, China.
Zhonghua Yan Ke Za Zhi. 2009 Oct;45(10):935-9.
To study the prognostic factors of retinoblastoma (RB).
Prospective case series study.With Envision System, surgical specimens from 31 RB cases were immunostained by using anti-NSE, GFAP and S-100 antibodies. Number of NSE positive cells was counted at 1000 tumor cells in each slice. The morphology of GFAP and S-100 stained positive cells were analyzed. All cases were followed-up for 5 to 17 years. Cox regression pattern was adopted to analyze the relationship between sex, age, stage, differentiation, number of NSE positive cells, the sizes of GFAP and S-100 positive cells, therapies and prognosis.
(1) Almost all cases were stained positively by anti-NSE antibodies. Positive rates of GFAP and S-100 expression specimens were 61.29% (19/31) and 58.06% (18/31), respectively. (2) The number of NSE positive cells was correlated with a better prognosis (P = 0.0361). The sizes of GFAP and S-100 stained area were also related with a better progress (P = 0.0430, 0.0477). In patients with a greater number of NSE stained cells (averaged 324/1000), the survival time was >/= 5 years. In patients with a less number of NSE stained cells (averaged 182/1000), the survival time was less than 5 years. (3) There was a significant correlation between the number of NSE stained cells and the results for GFAP and S-100 staining (r = 0.547, 0.581), respectively.
The number of NSE positive cells is correlated with a better prognosis of RB. The expression of GFAP and S-100 protein are also correlated with a better prognosis of RB. The number of NSE positive cells and the expression of GFAP and S-100 protein may be regarded as the standards in judging the degree of differentiation of RB, and may be used as one of criteria for the estimation of the prognosis.
研究视网膜母细胞瘤（RB）的预后因素。
前瞻性病例系列研究。采用Envision系统，用抗神经元特异性烯醇化酶（NSE）、胶质纤维酸性蛋白（GFAP）和S-100抗体对31例RB手术标本进行免疫染色。在每张切片的1000个肿瘤细胞中计数NSE阳性细胞数。分析GFAP和S-100染色阳性细胞的形态。所有病例随访5至17年。采用Cox回归模型分析性别、年龄、分期、分化程度、NSE阳性细胞数、GFAP和S-100阳性细胞大小、治疗方法与预后的关系。
（1）几乎所有病例抗NSE抗体染色均为阳性。GFAP和S-100表达标本的阳性率分别为61.29%（19/31）和58.06%（18/31）。（2）NSE阳性细胞数与较好的预后相关（P = 0.0361）。GFAP和S-100染色面积大小也与较好的病情进展相关（P = 0.0430，0.0477）。NSE染色细胞数较多（平均324/1000）的患者，生存时间≥5年。NSE染色细胞数较少（平均182/1000）的患者，生存时间小于5年。（3）NSE染色细胞数与GFAP和S-100染色结果之间分别存在显著相关性（r = 0.547，0.581）。
NSE阳性细胞数与RB较好的预后相关。GFAP和S-100蛋白的表达也与RB较好的预后相关。NSE阳性细胞数及GFAP和S-100蛋白的表达可作为判断RB分化程度的标准，也可作为评估预后的标准之一。
