Gu Yang-Kui, Huang Jin-Hua, Luo Rong-Guang, Gao Fei, Fan Wei-Jun, Zhang Liang
Department of Imaging and Interventional Radiology, State Key Laboratory of Oncology in Southern China, Department of Medical Imaging and Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
Zhonghua Yi Xue Za Zhi. 2009 Oct 27;89(39):2802-5.
To observe the effects of radiofrequency ablation in porcine liver ex vivo with "wet" RITA UniBlate unipolar electrode, evaluate the morphologic characteristic of the ablated zones and explore the impact upon the ablation lesions of time, temperature and set power of "wet" monopolar electrodes.
The maximal effective ablation electrode length of this "wet" unipolar electrode is 2.5 cm. According to ablation time, temperature and power respectively, the experiment was divided into three major groups of A, B, C (11 sub-groups). The investigators observed the morphological characteristics of ablation lesions, measured the longitudinal and transverse distance of ablation beyond the electrode tip and calculated the volume of ablation lesion. Statistical analysis was performed with one-way ANOVA upon the above-mentioned four parameters. And the pathological examinations were made with HE staining.
(1) All of ablated zones were ellipsoid. The cross-section of ablation lesions appeared to have 3 zones from the inside out: central zone, coagulated zone, hemorrhage and edema zone. (2) When ablating time and temperature increased to 15 min and 103 degrees C respectively, the ablated zones peaked: longitudinal diameter, transverse diameter, ablation distance beyond electrode tip and volume of each ablated zone was (3.49 +/- 0.09) cm, (2.40 +/- 0.13) cm, (0.79 +/- 0.09) cm and (10.6 +/- 1.3) cm(3) respectively. With power as a variable, different sizes between ablated zones showed no statistical significance. (3) The morphology of ablated zones became irregular when the electrodes were located around larger blood vessels.
The ablating time and temperature are the major factors of affecting the size of radiofrequency ablation lesion with RITA "wet" unipolar electrode.
观察“湿”性RITA单极电极在离体猪肝上进行射频消融的效果，评估消融灶的形态特征，并探讨“湿”性单极电极的时间、温度及设定功率对消融灶的影响。
该“湿”性单极电极的最大有效消融电极长度为2.5cm。根据消融时间、温度和功率分别将实验分为A、B、C三大组（11个亚组）。观察消融灶的形态特征，测量电极尖端以外消融的纵径和横径，并计算消融灶体积。对上述四个参数进行单因素方差分析。并用苏木精-伊红染色进行病理检查。
（1）所有消融区均为椭圆形。消融灶的横截面从内向外呈现3个区域：中心区、凝固区、出血和水肿区。（2）当消融时间和温度分别增加到15分钟和103℃时，消融区达到峰值：每个消融区的纵径、横径、电极尖端以外的消融距离和体积分别为（3.49±0.09）cm、（2.40±0.13）cm、（0.79±0.09）cm和（10.6±1.3）cm³。以功率为变量，消融区之间的不同大小无统计学意义。（3）当电极位于较大血管周围时，消融区形态变得不规则。
消融时间和温度是影响RITA“湿”性单极电极射频消融灶大小的主要因素。
