Yao Li-fen, Wang Zhen-kui, Wang Zhen-gang, Sui Dan, Zhang Li-ming
Department of Neurology, First Affiliated Hospital, Harbin Medical University, China.
Zhonghua Yi Xue Za Zhi. 2009 Nov 24;89(43):3058-60.
To study the expression of Cx32 and Cx43 in medically intractable temporal lobe epilepsy in human and investigate the pathogenic relationship between gap junctions and seizures.
The expression of Cx32 and Cx43 was detected by Western blot and immunohistochemistry in 14 consecutive samples of hippocampus from epileptic patients undergoing an amygdalohippocampectomy for the treatment of intractable seizures. During postmortem dissection, 8 samples of hippocampus in nonepileptic patients dying of other diseases were taken as control group.
The expression of Cx32 and Cx43 was at a low level in the control group [Cx32: count of positive cell (9.4 +/- 1.1), ratios of gray scale (0.2 +/- 0.1); Cx43: count of positive cell (9.2 +/- 4.7), ratios of gray scale (0.5 +/- 0.2)], but Cx43 and Cx32 appeared to be expressed at a higher level in epileptic patients compared with that of the control group by immunohistochemistry [Cx32: count of positive cell (14.6 +/- 3.4), Cx43: count of positive cell (16.5 +/- 3.1)] (P < 0.01), and their expression significantly increased by Western blot [Cx32: ratios of gray scale (1.5 +/- 0.2), Cx43: ratios of gray scale (1.4 +/- 0.3)] (P < 0.01). Over-expression of Cx32 and Cx43 was found in 14 consecutive samples of hippocampus from epileptic patients.
Gap junctions play an important role in the occurrence and progression of intractable seizures.
研究Cx32和Cx43在人类药物难治性颞叶癫痫中的表达，探讨缝隙连接与癫痫发作之间的致病关系。
采用蛋白质免疫印迹法和免疫组织化学法检测14例因药物难治性癫痫接受杏仁核海马切除术患者海马组织中Cx32和Cx43的表达。在尸检过程中，取8例死于其他疾病的非癫痫患者海马组织作为对照组。
对照组中Cx32和Cx43表达水平较低[Cx32：阳性细胞计数(9.4±1.1)，灰度比值(0.2±0.1)；Cx43：阳性细胞计数(9.2±4.7)，灰度比值(0.5±0.2)]，但免疫组织化学结果显示癫痫患者中Cx43和Cx32的表达水平高于对照组Cx32：阳性细胞计数(14.6±3.4)，Cx43：阳性细胞计数(16.5±3.1)，蛋白质免疫印迹法检测结果显示其表达显著增加Cx32：灰度比值(1.5±0.2)，Cx43：灰度比值(1.4±0.3)。在14例连续的癫痫患者海马组织样本中均发现Cx32和Cx43过表达。
缝隙连接在药物难治性癫痫发作的发生和发展中起重要作用。
