Tong Xu-Hui, Gu Yu-Chen, Jiao Hao, Yu Li, Dong Shu-Ying
Department of Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2015 Jan;44(1):67-73. doi: 10.3785/j.issn.1008-9292.2015.01.011.
To investigate the effects of inhibiting gap junctional intercellular communication on hypoxia/reoxygenation injury in astrocytes.
Primary cultured cerebral cortical astrocytes of neonate rats were divided into normal control group, hypoxia reoxygenation injury group and 18-α-glycyrrhetinic acid and oleamide (gap junctional intercellular channel inhibitors) group. The gap junction intercellular communication was determined by Parachute assay. The viability of astrocyes was detected by MTT assay. The apoptosis of astrocytes were detected with annexin V/PI and Hoechst 33258 staining.
Compared with the normal control group, the gap junctional function of astrocytes was increased significantly in ischemia/reperfusion group (P<0.01), the surviving fraction of astrocytes decreased significantly (P<0.01) and its cell apoptosis ratio increased significantly (P<0.01). Compared with the ischemia/reperfusion group, the gap junctional function of astrocytes in18-α-glycyrrhetinic acid and oleamide group decreased significantly (P<0.01), the viability of astrocytes increased significantly (P<0.01), while cell apoptosis decreased significantly (P<0.01).
Inhibition of intercellular gap junction has protective effect against hypoxia/reoxygenation injury in astrocytes.
探讨抑制缝隙连接细胞间通讯对星形胶质细胞缺氧/复氧损伤的影响。
将新生大鼠原代培养的大脑皮质星形胶质细胞分为正常对照组、缺氧复氧损伤组和18-α-甘草次酸与油酰胺（缝隙连接细胞间通道抑制剂）组。采用降落伞试验测定缝隙连接细胞间通讯。用MTT法检测星形胶质细胞的活力。用膜联蛋白V/碘化丙啶及Hoechst 33258染色检测星形胶质细胞的凋亡情况。
与正常对照组比较，缺血/再灌注组星形胶质细胞缝隙连接功能显著增强（P<0.01），星形胶质细胞存活分数显著降低（P<0.01），细胞凋亡率显著升高（P<0.01）。与缺血/再灌注组比较，18-α-甘草次酸与油酰胺组星形胶质细胞缝隙连接功能显著降低（P<0.01），星形胶质细胞活力显著升高（P<0.01），细胞凋亡显著减少（P<0.01）。
抑制细胞间缝隙连接对星形胶质细胞缺氧/复氧损伤具有保护作用。
