Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, Guangxi University, Nanning, PR China.
Anim Reprod Sci. 2010 Jun;119(3-4):169-71. doi: 10.1016/j.anireprosci.2010.01.001. Epub 2010 Jan 14.
Flow cytometry sorting of X- and Y-chromosome bearing sperm has been emerging as a promising technology to alter the sex ratio in progenies of mammals in the recent years. The objective of this study was to evaluate the efficiency of AI by using the sexed sperm to produce sex-preselected calves in buffalo species. A total of 43 buffalo cows were inseminated with X-sorted sperm, 30 of which were confirmed pregnant 3 mo following AI. In terms of conception rate, significant difference was observed between AI with sexed sperm derived from different bulls (P<0.05), but not between sexed and non-sexed sperm (P>0.05), nor between heifers and parous buffalo cows (P>0.05). A total of 29 sex-preselected calves, 24 females and 5 males, developed to term and were viable on delivery. Results of this study indicate the feasibility of the application of the sexing technology to accelerate the genetic improvement in swamp buffalo.
近年来，流式细胞术分选携带有 X 染色体和 Y 染色体的精子已成为改变哺乳动物后代性别比例的一种很有前途的技术。本研究的目的是评估使用分选精子生产水牛品种性别预选小牛的效率。共有 43 头母水牛用 X 染色体分选精子进行人工授精，其中 30 头在人工授精后 3 个月确认怀孕。在受孕率方面，来自不同公牛的分选精子的人工授精之间存在显著差异（P<0.05），但分选精子和非分选精子之间（P>0.05）、小母牛和经产母水牛之间（P>0.05）均无显著差异。共有 29 头性别预选小牛足月发育并存活分娩。本研究结果表明，应用性别鉴定技术加速沼泽水牛遗传改良是可行的。
