Price Claire E, Driessen Arnold J M
Department of Molecular Microbiology, University of Groningen, 9751 NN Haren, The Netherlands.
Biochim Biophys Acta. 2010 Jun;1803(6):748-66. doi: 10.1016/j.bbamcr.2010.01.019. Epub 2010 Feb 4.
Escherichia coli is one of the preferred bacteria for studies on the energetics and regulation of respiration. Respiratory chains consist of primary dehydrogenases and terminal reductases or oxidases linked by quinones. In order to assemble this complex arrangement of protein complexes, synthesis of the subunits occurs in the cytoplasm followed by assembly in the cytoplasm and/or membrane, the incorporation of metal or organic cofactors and the anchoring of the complex to the membrane. In the case of exported metalloproteins, synthesis, assembly and incorporation of metal cofactors must be completed before translocation across the cytoplasmic membrane. Coordination data on these processes is, however, scarce. In this review, we discuss the various processes that respiratory proteins must undergo for correct assembly and functional coupling to the electron transport chain in E. coli. Targeting to and translocation across the membrane together with cofactor synthesis and insertion are discussed in a general manner followed by a review of the coordinated biogenesis of individual respiratory enzyme complexes. Lastly, we address the supramolecular organization of respiratory enzymes into supercomplexes and their localization to specialized domains in the membrane.
大肠杆菌是研究呼吸作用的能量学和调节机制时首选的细菌之一。呼吸链由通过醌连接的初级脱氢酶和末端还原酶或氧化酶组成。为了组装这种蛋白质复合物的复杂排列，亚基在细胞质中合成，随后在细胞质和/或膜中组装，金属或有机辅因子的掺入以及复合物与膜的锚定。对于输出的金属蛋白，金属辅因子的合成、组装和掺入必须在跨细胞质膜转运之前完成。然而，关于这些过程的协调数据很少。在这篇综述中，我们讨论了呼吸蛋白在大肠杆菌中为正确组装并与电子传递链进行功能偶联而必须经历的各种过程。以一般方式讨论了靶向和跨膜转运以及辅因子合成和插入，随后综述了各个呼吸酶复合物的协调生物合成。最后，我们探讨了呼吸酶超分子组装成超复合物及其在膜中特定区域的定位。
