Biotoxin Unit of the Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
Free Radic Biol Med. 2010 May 1;48(9):1173-81. doi: 10.1016/j.freeradbiomed.2010.01.036. Epub 2010 Feb 4.
The question of how amphibians can protect themselves from reactive oxygen species when exposed to the sun in an oxygen-rich atmosphere is important and interesting, not only from an evolutionary viewpoint, but also as a primer for researchers interested in mammalian skin biology, in which such peptide systems for antioxidant defense are not well studied. The identification of an antioxidant peptide named antioxidin-RL from frog (Odorrana livida) skin in this report supports the idea that a peptide antioxidant system may be a widespread antioxidant strategy among amphibian skins. Its ability to eliminate most of the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical tested within 2 s, which is much faster than the commercial antioxidant factor butylated hydroxytoluene, suggests that it has a potentially large impact on redox homeostasis in amphibian skins. Cys10 is proven to be responsible for its rapid radical scavenging function and tyrosines take part in the binding of antioxidin-RL to radicals according to our nuclear magnetic resonance assay.
当暴露在富含氧气的大气中的阳光下时，两栖动物如何保护自己免受活性氧的伤害，这不仅从进化的角度来看是一个重要且有趣的问题，而且对于研究哺乳动物皮肤生物学的研究人员来说也是一个入门，因为在哺乳动物皮肤生物学中，这种肽系统的抗氧化防御作用尚未得到很好的研究。本报告从青蛙（Odorrana livida）皮肤中鉴定出一种名为抗氧化肽-RL 的抗氧化肽，支持了这样一种观点，即肽抗氧化系统可能是两栖动物皮肤中广泛存在的抗氧化策略。它在 2 秒内消除了大部分 2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）自由基的能力，比商业抗氧化剂丁羟甲苯快得多，这表明它对两栖动物皮肤的氧化还原平衡有潜在的重大影响。根据我们的核磁共振分析，Cys10 被证明负责其快速清除自由基的功能，而酪氨酸参与了抗氧化肽-RL 与自由基的结合。
