Key Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, Shandong, China.
Key Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, Shandong, China; Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, 650223, Yunnan, China; Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, China.
Peptides. 2018 Mar;101:124-134. doi: 10.1016/j.peptides.2018.01.009. Epub 2018 Jan 16.
The unavoidable daily exposure of the skin to ultraviolet (UV) B radiation is proven to have deleterious effects. The action mechanism of antioxidin-RL, an antioxidant peptide purified from skin secretions of frog Odorrana livida with amino acid sequence of AMRLTYNRPCIYAT, is well characterized by NMR titration and mutation based on ABTS scavenging activities. In order to explore the protective effects of antioxidin-RL against UVB-irradiated skin photoaging, cell uptake assay was used to detect the location of antioxidin-RL molecules serving various biological functions in the cells. The protective effects of antioxidin-RL on UVB-induced response were examined in vitro and in vivo. Results showed that antioxidin-RL successfully penetrated the cell membrane and exerted a positive effect on cell migration. It also effectively inhibited the UVB-induced excessive production of ROS and prevented oxidative damage to DNAs and proteins. Moreover, the mRNA expressions of MMP-1, VEGF, COX-2, and pro-inflammatory cytokines, such as IL-6 and TNF-α in antioxidin-RL-treated HaCaT and HSF cells were significantly down-regulated whereas those of FGF, procollagen type I and TGF-β1 up-regulated. Antioxidin-RL effectively prevented UVB-induced erythema on mouse skin, thereby inhibiting UVB-induced skin thickening and inflammation and increasing collagen deposition as demonstrated by in vivo experiments. Hence, the novel antioxidant peptide antioxidin-RL can effectively reduce UVB-induced skin reactions in vivo and in vitro, providing potential molecules against UVB-induced inflammation and photoaging.
皮肤每天不可避免地暴露在紫外线 (UV) B 辐射下，已被证明会产生有害影响。从 Odorrana livida 皮肤分泌物中纯化出的具有氨基酸序列 AMRLTYNRPCIYAT 的抗氧化肽——抗氧化素-RL 的作用机制已通过 NMR 滴定和基于 ABTS 清除活性的突变得到很好的描述。为了探索抗氧化素-RL 对 UVB 照射皮肤光老化的保护作用，采用细胞摄取试验检测了在细胞中具有各种生物学功能的抗氧化素-RL 分子的位置。在体外和体内研究了抗氧化素-RL 对 UVB 诱导反应的保护作用。结果表明，抗氧化素-RL 成功穿透细胞膜，并对细胞迁移产生积极影响。它还能有效抑制 ROS 的过量产生，防止 DNA 和蛋白质的氧化损伤。此外，在经抗氧化素-RL 处理的 HaCaT 和 HSF 细胞中，MMP-1、VEGF、COX-2 和促炎细胞因子（如 IL-6 和 TNF-α）的 mRNA 表达显著下调，而 FGF、I 型前胶原和 TGF-β1 的 mRNA 表达上调。抗氧化素-RL 能有效预防小鼠皮肤的 UVB 诱导红斑，从而抑制 UVB 诱导的皮肤增厚和炎症，并通过体内实验增加胶原蛋白沉积。因此，新型抗氧化肽抗氧化素-RL 可有效减少体内和体外的 UVB 诱导皮肤反应，为防治 UVB 诱导的炎症和光老化提供潜在的分子。
