• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

布瑞宁-1FL 肽对卡拉胶诱导损伤急性炎症模型中氧化应激效应的多种作用机制。

Multiple Mechanistic Action of Brevinin-1FL Peptide against Oxidative Stress Effects in an Acute Inflammatory Model of Carrageenan-Induced Damage.

机构信息

Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.

出版信息

Oxid Med Cell Longev. 2022 Sep 5;2022:2615178. doi: 10.1155/2022/2615178. eCollection 2022.

DOI:10.1155/2022/2615178
PMID:36105482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9467757/
Abstract

Amphibian skin is acknowledged to contain an antioxidant system composed of various gene-encoded antioxidant peptides, which exert significant effects on host defense. Nevertheless, recognition of such peptides is in its infancy so far. Here, we reported the antioxidant properties and underlying mechanism of a new antioxidant peptide, brevinin-1FL, identified from frog skin. The cDNA sequence encoding brevinin-1FL was successfully cloned from the total cDNA of and showed to contain 222 bp. The deduced mature peptide sequence of brevinin-1FL was FWERCSRWLLN. Functional analysis revealed that brevinin-1FL could concentration-dependently scavenge ABTS, DPPH, NO, and hydroxyl radicals and alleviate iron oxidation. Besides, brevinin-1FL was found to show neuroprotective activity by reducing contents of MDA and ROS plus mitochondrial membrane potential, increasing endogenous antioxidant enzyme activity, and suppressing HO-induced death, apoptosis, and cycle arrest in PC12 cells which were associated with its regulation of AKT/MAPK/NF-B signal pathways. Moreover, brevinin-1FL relieved paw edema, decreased the levels of TNF-, IL-1, IL-6, MPO, and malondialdehyde (MDA), and restored catalase (CAT) and superoxide dismutase (SOD) activity plus glutathione (GSH) contents in the mouse injected by carrageenan. Together, these findings indicate that brevinin-1FL as an antioxidant has potent therapeutic potential for the diseases induced by oxidative damage. Meanwhile, this study will help us further comprehend the biological functions of amphibian skin and the mechanism by which antioxidants protect cells from oxidative stress.

摘要

两栖动物皮肤被认为含有由各种基因编码的抗氧化肽组成的抗氧化系统，这些肽对宿主防御具有重要作用。然而，到目前为止，人们对这些肽的认识还处于起步阶段。在这里，我们报道了一种从青蛙皮肤中鉴定出的新型抗氧化肽——brevienin-1FL 的抗氧化特性及其潜在机制。成功地从 的总 cDNA 中克隆了编码 brevinin-1FL 的 cDNA 序列，该序列包含 222bp。brevienin-1FL 的推导成熟肽序列为 FWERCSRWLLN。功能分析表明，brevienin-1FL 可以浓度依赖性地清除 ABTS、DPPH、NO 和羟基自由基，并减轻铁氧化。此外，brevienin-1FL 被发现通过降低 MDA 和 ROS 加线粒体膜电位的含量、增加内源性抗氧化酶的活性以及抑制 HO 诱导的 PC12 细胞死亡、凋亡和周期停滞来表现出神经保护活性，这与其调节 AKT/MAPK/NF-B 信号通路有关。此外，brevienin-1FL 缓解了爪肿胀，降低了角叉菜胶注射小鼠中 TNF-、IL-1、IL-6、MPO 和丙二醛 (MDA) 的水平，并恢复了过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 的活性以及谷胱甘肽 (GSH) 的含量。总之，这些发现表明，作为一种抗氧化剂，brevienin-1FL 具有治疗由氧化损伤引起的疾病的潜在治疗潜力。同时，本研究将帮助我们进一步了解两栖动物皮肤的生物学功能以及抗氧化剂保护细胞免受氧化应激的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/c7e59fbf8b32/OMCL2022-2615178.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/df67e165fb0f/OMCL2022-2615178.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/08b4aaea8ff1/OMCL2022-2615178.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/69ce50aae8c2/OMCL2022-2615178.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/b1068f523b11/OMCL2022-2615178.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/1290a0efc0ae/OMCL2022-2615178.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/b75f9aec23cf/OMCL2022-2615178.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/2aceddb8ea7e/OMCL2022-2615178.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/c7e59fbf8b32/OMCL2022-2615178.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/df67e165fb0f/OMCL2022-2615178.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/08b4aaea8ff1/OMCL2022-2615178.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/69ce50aae8c2/OMCL2022-2615178.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/b1068f523b11/OMCL2022-2615178.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/1290a0efc0ae/OMCL2022-2615178.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/b75f9aec23cf/OMCL2022-2615178.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/2aceddb8ea7e/OMCL2022-2615178.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbfe/9467757/c7e59fbf8b32/OMCL2022-2615178.008.jpg

相似文献

1
Multiple Mechanistic Action of Brevinin-1FL Peptide against Oxidative Stress Effects in an Acute Inflammatory Model of Carrageenan-Induced Damage.布瑞宁-1FL 肽对卡拉胶诱导损伤急性炎症模型中氧化应激效应的多种作用机制。
Oxid Med Cell Longev. 2022 Sep 5;2022:2615178. doi: 10.1155/2022/2615178. eCollection 2022.
2
Novel antimicrobial peptides isolated from the skin secretions of Hainan odorous frog, Odorrana hainanensis.从海南臭蛙 Odorrana hainanensis 的皮肤分泌物中分离出的新型抗菌肽。
Peptides. 2012 Jun;35(2):285-90. doi: 10.1016/j.peptides.2012.03.007. Epub 2012 Mar 16.
3
Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata.两种新型抗菌肽来自青蛙皮肤分泌物，黑眶蟾蜍。
J Pept Sci. 2011 Jan;17(1):68-72. doi: 10.1002/psc.1309. Epub 2010 Oct 25.
4
Host defense peptides from Lithobates forreri, Hylarana luctuosa, and Hylarana signata (Ranidae): phylogenetic relationships inferred from primary structures of ranatuerin-2 and brevinin-2 peptides.来自福氏蛙、哀鸣树蛙和饰纹树蛙（蛙科）的宿主防御肽：基于蛙皮素 -2 和铃蟾肽 -2 肽一级结构推断的系统发育关系
Comp Biochem Physiol Part D Genomics Proteomics. 2014 Mar;9:49-57. doi: 10.1016/j.cbd.2014.01.002. Epub 2014 Jan 11.
5
Cytotoxic peptides with insulin-releasing activities from skin secretions of the Italian stream frog Rana italica (Ranidae).来自意大利溪流蛙（蛙科）皮肤分泌物中具有胰岛素释放活性的细胞毒性肽。
J Pept Sci. 2017 Oct;23(10):769-776. doi: 10.1002/psc.3025. Epub 2017 Jul 11.
6
Identification and characterization of antimicrobial peptides from skin of Amolops ricketti (Anura: Ranidae).鉴定和表征中华大蟾蜍（无尾目：蟾蜍科）皮肤中的抗菌肽。
Peptides. 2012 Jan;33(1):27-34. doi: 10.1016/j.peptides.2011.10.030. Epub 2011 Nov 11.
7
Brevinin-2PN, an antimicrobial peptide identified from dark-spotted frog (Pelophylax nigromaculatus), exhibits wound-healing activity.Brevinin-2PN，一种从黑斑蛙（Pelophylax nigromaculatus）中鉴定出的抗菌肽，具有促进伤口愈合的活性。
Dev Comp Immunol. 2022 Dec;137:104519. doi: 10.1016/j.dci.2022.104519. Epub 2022 Aug 27.
8
Isolation, characterization and molecular cloning of new antimicrobial peptides belonging to the brevinin-1 and temporin families from the skin of Hylarana latouchii (Anura: Ranidae).从叶氏蛙（无尾目：蛙科）皮肤中分离、鉴定及分子克隆属于brevinin-1和temporin家族的新型抗菌肽
Biochimie. 2009 Apr;91(4):540-7. doi: 10.1016/j.biochi.2009.01.007.
9
Antimicrobial and Anti-inflammatory Effects of a Novel Peptide From the Skin of Frog .一种源自青蛙皮肤的新型肽的抗菌和抗炎作用
Front Pharmacol. 2021 Dec 16;12:783108. doi: 10.3389/fphar.2021.783108. eCollection 2021.
10
A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae).从亚洲蛙（贡氏蛙，无尾目：蛙科）皮肤提取物中分离出的一种强效、无毒的胰岛素释放肽。
Regul Pept. 2008 Nov 29;151(1-3):153-9. doi: 10.1016/j.regpep.2008.04.002. Epub 2008 Apr 13.

引用本文的文献

1
The Role of Amphibian AMPs Against Oxidative Stress and Related Diseases.两栖动物抗菌肽在抵抗氧化应激及相关疾病中的作用。
Antibiotics (Basel). 2025 Jan 25;14(2):126. doi: 10.3390/antibiotics14020126.
2
Cathelicidin-HG Alleviates Sepsis-Induced Platelet Dysfunction by Inhibiting GPVI-Mediated Platelet Activation.抗菌肽-HG通过抑制糖蛋白VI介导的血小板活化减轻脓毒症诱导的血小板功能障碍。
Research (Wash D C). 2024 Jun 5;7:0381. doi: 10.34133/research.0381. eCollection 2024.
3
Chensinin-1b Alleviates DSS-Induced Inflammatory Bowel Disease by Inducing Macrophage Switching from the M1 to the M2 Phenotype.

本文引用的文献

1
Fejerlectin, a Lectin-like Peptide from the Skin of , Inhibits HIV-1 Entry by Targeting Gp41.费耶尔凝集素，一种源自[生物名称]皮肤的凝集素样肽，通过靶向糖蛋白41抑制HIV-1进入。
ACS Omega. 2021 Feb 24;6(9):6414-6423. doi: 10.1021/acsomega.1c00033. eCollection 2021 Mar 9.
2
Reactive Oxygen Species: Drivers of Physiological and Pathological Processes.活性氧：生理和病理过程的驱动因素
J Inflamm Res. 2020 Dec 2;13:1057-1073. doi: 10.2147/JIR.S275595. eCollection 2020.
3
Neuroprotective Effect of Antioxidants in the Brain.抗氧化剂对大脑的神经保护作用。
人参皂苷Chensinin-1b通过诱导巨噬细胞从M1型向M2型表型转换减轻葡聚糖硫酸钠诱导的炎症性肠病
Biomedicines. 2024 Feb 1;12(2):345. doi: 10.3390/biomedicines12020345.
4
New Insights into Antioxidant Peptides: An Overview of Efficient Screening, Evaluation Models, Molecular Mechanisms, and Applications.抗氧化肽的新见解：高效筛选、评估模型、分子机制及应用概述
Antioxidants (Basel). 2024 Feb 5;13(2):203. doi: 10.3390/antiox13020203.
5
Cath-KP, a novel peptide derived from frog skin, prevents oxidative stress damage in a Parkinson's disease model.Cath-KP，一种源自青蛙皮肤的新型肽，可预防帕金森病模型中的氧化应激损伤。
Zool Res. 2024 Jan 18;45(1):108-124. doi: 10.24272/j.issn.2095-8137.2023.101.
Int J Mol Sci. 2020 Sep 28;21(19):7152. doi: 10.3390/ijms21197152.
4
Enhanced Antimicrobial Activity of N-Terminal Derivatives of a Novel Brevinin-1 Peptide from The Skin Secretion of .新型蛙皮素 1 肽 N 端衍生物的增强抗菌活性。
Toxins (Basel). 2020 Jul 30;12(8):484. doi: 10.3390/toxins12080484.
5
Antioxidant properties and neuroprotective effects of Esc-1GN through the regulation of MAPK and AKT signaling.通过调节 MAPK 和 AKT 信号通路，Esc-1GN 表现出抗氧化特性和神经保护作用。
Life Sci. 2020 Aug 1;254:117753. doi: 10.1016/j.lfs.2020.117753. Epub 2020 May 6.
6
Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.迷迭香酸通过调节Nrf2/HO-1和TLR4/NF-κB信号通路抑制氧化应激和炎症反应，从而对脊髓损伤发挥神经保护作用。
Toxicol Appl Pharmacol. 2020 Apr 19;397:115014. doi: 10.1016/j.taap.2020.115014.
7
The Antioxidant Peptide Salamandrin-I: First Bioactive Peptide Identified from Skin Secretion of Salamandra Genus (Salamandra salamandra).抗氧化肽 Salamandrin-I：首次从 Salamandra 属（Salamandra salamandra）皮肤分泌物中鉴定出的生物活性肽。
Biomolecules. 2020 Mar 27;10(4):512. doi: 10.3390/biom10040512.
8
Role of Reactive Oxygen Species in Cancer Progression: Molecular Mechanisms and Recent Advancements.活性氧在癌症进展中的作用：分子机制与最新进展。
Biomolecules. 2019 Nov 13;9(11):735. doi: 10.3390/biom9110735.
9
LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, .LFB：一种新型抗菌 Brevinin 样肽，来自福建大头蛙的皮肤分泌物。
Biomolecules. 2019 Jun 21;9(6):242. doi: 10.3390/biom9060242.
10
Ethanol Extract of Exerts Antioxidant and Neuroprotective Effects via Activation of the Nrf2 Signaling Pathway.乙醇提取物通过激活 Nrf2 信号通路发挥抗氧化和神经保护作用。
Oxid Med Cell Longev. 2019 Apr 3;2019:9421037. doi: 10.1155/2019/9421037. eCollection 2019.