Department of Pharmaceutical Technology, Jadavpur University 188, Raja S.C. Mallick Road, P.O. Box 17028, Kolkata 700032, West Bengal, India.
Clin Nutr. 2010 Aug;29(4):531-7. doi: 10.1016/j.clnu.2009.12.012.
BACKGROUND & AIMS: The aim of this study is to assess the effect of dietary fish oil (MaxEPA) on DNA-strand breaks, cell proliferation and anti-apoptotic protein expressions in rat mammary carcinogenesis.
Eighty-one female Sprague-Dawley rats were divided into two parts, one for DNA-strand breaks study and the other for immunohistochemical study. Female Sprague-Dawley rats were treated with 7,12-dimethylbenz(alpha)anthracene (DMBA) (0.5 mg/0.2 ml corn oil/100 g body weight) by a tail vein injection. Rats were fed either fish oil or corn oil (0.5 ml/day/rat) by oral gavage.
Fish oil-treated group showed significant protection against generation of single-strand breaks (SSBs) (56.1%, P < 0.05) but increased effect (72.3%, P < 0.05) was found in the corn oil-treated group when compared to DMBA control group. Furthermore, fish oil-treated group exhibited substantial decrease in Ki-67 (P < 0.05), HER-2/neu (P < 0.05) and c-Myc (P < 0.05) immunolabelling indices when compared to carcinogen counterpart. However, corn oil treatment resulted in significant increase in the above parameters.
The above data support the role of n-3 PUFA as a preventive agent for DNA damages and a potential to inhibit mammary carcinogenesis.
本研究旨在评估饮食中海鱼油（MaxEPA）对大鼠乳腺癌发生过程中 DNA 链断裂、细胞增殖和抗凋亡蛋白表达的影响。
81 只雌性 Sprague-Dawley 大鼠分为两部分，一部分用于 DNA 链断裂研究，另一部分用于免疫组织化学研究。雌性 Sprague-Dawley 大鼠经尾静脉注射 7,12-二甲基苯并（α）蒽（DMBA）（0.5mg/0.2ml 玉米油/100g 体重）。大鼠通过口服灌胃分别给予鱼油或玉米油（0.5ml/天/只）。
与 DMBA 对照组相比，鱼油组单链断裂（SSB）的产生明显受到保护（56.1%，P<0.05），但玉米油组的保护作用增强（72.3%，P<0.05）。此外，与致癌剂对照组相比，鱼油组 Ki-67（P<0.05）、HER-2/neu（P<0.05）和 c-Myc（P<0.05）免疫标记指数显著降低。然而，玉米油处理导致上述参数显著增加。
上述数据支持 n-3PUFA 作为 DNA 损伤预防剂和抑制乳腺癌发生的潜在作用。
