鱼油可调节细胞增殖，保护 DNA 损伤，并降低大鼠乳腺癌发生过程中的 HER-2/neu 和 c-Myc 蛋白表达。

Fish oil regulates cell proliferation, protect DNA damages and decrease HER-2/neu and c-Myc protein expression in rat mammary carcinogenesis.

机构信息

Department of Pharmaceutical Technology, Jadavpur University 188, Raja S.C. Mallick Road, P.O. Box 17028, Kolkata 700032, West Bengal, India.

出版信息

Clin Nutr. 2010 Aug;29(4):531-7. doi: 10.1016/j.clnu.2009.12.012.

DOI:10.1016/j.clnu.2009.12.012

PMID:20138412

Abstract

BACKGROUND & AIMS: The aim of this study is to assess the effect of dietary fish oil (MaxEPA) on DNA-strand breaks, cell proliferation and anti-apoptotic protein expressions in rat mammary carcinogenesis.

METHODS

Eighty-one female Sprague-Dawley rats were divided into two parts, one for DNA-strand breaks study and the other for immunohistochemical study. Female Sprague-Dawley rats were treated with 7,12-dimethylbenz(alpha)anthracene (DMBA) (0.5 mg/0.2 ml corn oil/100 g body weight) by a tail vein injection. Rats were fed either fish oil or corn oil (0.5 ml/day/rat) by oral gavage.

RESULTS

Fish oil-treated group showed significant protection against generation of single-strand breaks (SSBs) (56.1%, P < 0.05) but increased effect (72.3%, P < 0.05) was found in the corn oil-treated group when compared to DMBA control group. Furthermore, fish oil-treated group exhibited substantial decrease in Ki-67 (P < 0.05), HER-2/neu (P < 0.05) and c-Myc (P < 0.05) immunolabelling indices when compared to carcinogen counterpart. However, corn oil treatment resulted in significant increase in the above parameters.

CONCLUSIONS

The above data support the role of n-3 PUFA as a preventive agent for DNA damages and a potential to inhibit mammary carcinogenesis.

摘要

背景与目的

本研究旨在评估饮食中海鱼油（MaxEPA）对大鼠乳腺癌发生过程中 DNA 链断裂、细胞增殖和抗凋亡蛋白表达的影响。

方法

81 只雌性 Sprague-Dawley 大鼠分为两部分，一部分用于 DNA 链断裂研究，另一部分用于免疫组织化学研究。雌性 Sprague-Dawley 大鼠经尾静脉注射 7,12-二甲基苯并（α）蒽（DMBA）（0.5mg/0.2ml 玉米油/100g 体重）。大鼠通过口服灌胃分别给予鱼油或玉米油（0.5ml/天/只）。

结果

与 DMBA 对照组相比，鱼油组单链断裂（SSB）的产生明显受到保护（56.1%，P<0.05），但玉米油组的保护作用增强（72.3%，P<0.05）。此外，与致癌剂对照组相比，鱼油组 Ki-67（P<0.05）、HER-2/neu（P<0.05）和 c-Myc（P<0.05）免疫标记指数显著降低。然而，玉米油处理导致上述参数显著增加。