Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, Permoserstrasse 15, 04318 Leipzig, Germany.
Curr Opin Biotechnol. 2010 Feb;21(1):100-13. doi: 10.1016/j.copbio.2010.01.002.
Heterogeneity of industrial production cultures is accepted to a certain degree; however, the underlying mechanisms are seldom perceived or included in the development of new bioprocess control strategies. Population heterogeneity and its basics, perceptible in the diverse proficiency of cells, begins with asymmetric birth and is found to recess during the life cycle. Since inefficient subpopulations have significant impact on the productivity of industrial cultures, cellular heterogeneity needs to be detected and quantified by using high speed detection tools like flow cytometry. Possible origins of population heterogeneity, sophisticated fluorescent techniques for detection of individual cell states, and cutting-edge Omics-technologies for extended information beyond the resolution of fluorescent labelling are highlighted.
工业生产文化的异质性在一定程度上是被接受的；然而，潜在的机制很少被察觉，也很少被纳入新的生物过程控制策略的发展中。种群异质性及其基础，在细胞的不同能力中是显而易见的，它始于不对称的出生，并在生命周期中被发现是隐性的。由于低效的亚群对工业培养物的生产力有重大影响，因此需要使用高速检测工具（如流式细胞术）来检测和量化细胞异质性。本文强调了种群异质性的可能起源、用于检测单个细胞状态的复杂荧光技术，以及超越荧光标记分辨率的扩展信息的前沿组学技术。
