西印度群岛圣基茨流浪猫中的猫免疫缺陷病毒、猫白血病病毒和巴尔通体属细菌
Feline immunodeficiency virus, feline leukemia virus and Bartonella species in stray cats on St Kitts, West Indies.
作者信息
Kelly Patrick J, Moura Lenita, Miller Tanya, Thurk Jaime, Perreault Nicole, Weil Adriana, Maggio Ricardo, Lucas Helene, Breitschwerdt Edward
机构信息
Ross University School of Veterinary Medicine, PO Box 334, West Farm, Saint Kitts and Nevis.
出版信息
J Feline Med Surg. 2010 Jun;12(6):447-50. doi: 10.1016/j.jfms.2009.12.015. Epub 2010 Feb 6.
Stray cats trapped in various areas of Basseterre, the capital of St Kitts in the West Indies, were tested for infection with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) using commercial kits. Of 99 (51 male and 48 female) cats trapped in 2006/7, 15% (12 males and three females) were positive for FIV while none were positive for FeLV. Of 72 (41 males and 31 females) cats trapped in 2009, 14% (nine males and one female) were positive for FIV while none were positive for FeLV. Polymerase chain reaction analysis revealed DNA of Bartonella species in whole blood collected from 60/95 (63%) cats trapped in 2006/7. Sequencing of the 16S-23S rRNA gene intergenic transcribed spacer (ITS) region of a convenience sample of nine amplicons and the 11 isolates made from 43 blood samples which were cultured using Bartonella alpha Proteobacteria (BAPGM) enrichment medium revealed B henselae (14) and B clarridgeiae (six).
在西印度群岛圣基茨岛首都巴斯特尔的各个区域捕获的流浪猫，使用商用试剂盒检测是否感染猫免疫缺陷病毒（FIV）和猫白血病病毒（FeLV）。在2006/7年捕获的99只猫（51只雄性和48只雌性）中，15%（12只雄性和3只雌性）FIV呈阳性，而FeLV均为阴性。在2009年捕获的72只猫（41只雄性和31只雌性）中，14%（9只雄性和1只雌性）FIV呈阳性，FeLV同样均为阴性。聚合酶链反应分析显示，在2006/7年捕获的95只猫中的60只（63%）采集的全血中存在巴尔通体属DNA。对9个扩增子的便利样本以及从43份使用巴尔通体α变形杆菌（BAPGM）富集培养基培养的血样中分离出的11株菌株的16S - 23S rRNA基因间隔转录间隔区（ITS）进行测序，结果显示有亨氏巴尔通体（14株）和克拉氏巴尔通体（6株）。
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