National Center for Cell Science, University of Pune Campus, Pune 411007, India.
Biochem Biophys Res Commun. 2010 Mar 12;393(3):377-83. doi: 10.1016/j.bbrc.2010.01.126. Epub 2010 Feb 6.
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into multiple cell lineages. Presently, bone marrow is considered as a prime source of MSCs; however, there are some drawbacks and limitations in use of these MSCs for cell therapy. In this study, we demonstrate that human gingival tissue-derived MSCs have several advantages over bone marrow-derived MSCs. Gingival MSCs are easy to isolate, homogenous and proliferate faster than bone marrow MSCs without any growth factor. Importantly, gingival MSCs display stable morphology and do not loose MSC characteristic at higher passages. In addition, gingival MSCs maintain normal karyotype and telomerase activity in long-term cultures, and are not tumorigenic. Thus, we reveal that human gingiva is a better source of MSCs than bone marrow, and large number of functionally competent clinical grade MSCs can be generated in short duration for cell therapy in regenerative medicine and tissue engineering.
间充质干细胞（MSCs）具有自我更新和分化为多种细胞谱系的能力。目前，骨髓被认为是 MSCs 的主要来源；然而，在细胞治疗中使用这些 MSCs 存在一些缺点和限制。在这项研究中，我们证明了人牙龈组织来源的 MSCs 比骨髓来源的 MSCs 具有几个优势。牙龈 MSCs 易于分离，均一性好，增殖速度比骨髓 MSCs 快，无需任何生长因子。重要的是，牙龈 MSCs 在较高传代时保持稳定的形态，不会失去 MSC 特征。此外，牙龈 MSCs 在长期培养中保持正常核型和端粒酶活性，并且无致瘤性。因此，我们揭示了人牙龈是比骨髓更好的 MSCs 来源，并且可以在短时间内生成大量功能齐全的临床级 MSCs，用于再生医学和组织工程中的细胞治疗。
