Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Exp Parasitol. 2010 Jul;125(3):264-70. doi: 10.1016/j.exppara.2010.01.022. Epub 2010 Feb 6.
The effects of diclazuril on mRNA expression levels of invasion-related microneme genes were examined in second-generation merozoites of Eimeria tenella (E. tenella) by quantitative real-time (QRT) PCR. Diclazruil treatment of infected chickens significantly decreased the number of second-generation merozoites by 65.13%, and resulted in downregulation of EtMIC genes: EtMIC1 by 65.63%, EtMIC2 by 64.12%, EtMIC3 by 56.82%, EtMIC4 by 73.48%, and EtMIC5 by 78.17%. SEM images of caecum tissue from uninfected chickens showed regular intestinal villus structure. In infected chickens, a distinct loss of the superficial epithelium, with a flattened mucosa and large-area necrosis and anabrosis, was evident. In diclazruil-treated chickens, a decrease in merozoite number and a visibly improved appearance of the caeca were noted. These improvements appeared to be mediated in part by downregulation of the expression of invasion-related EtMIC genes in response to diclazuril.
通过定量实时（QRT）PCR 检测地克珠利对柔嫩艾美耳球虫（Eimeria tenella）第二代裂殖子侵袭相关微线体基因 mRNA 表达水平的影响。地克珠利处理感染鸡可使第二代裂殖子数量显著减少 65.13%，并导致 EtMIC 基因下调：EtMIC1 下调 65.63%，EtMIC2 下调 64.12%，EtMIC3 下调 56.82%，EtMIC4 下调 73.48%，EtMIC5 下调 78.17%。未感染鸡盲肠组织的 SEM 图像显示出正常的肠绒毛结构。在感染鸡中，明显失去了表面上皮，黏膜扁平，大面积坏死和再生。在用地克珠利处理的鸡中，裂殖子数量减少，盲肠外观明显改善。这些改善似乎部分是通过地克珠利对侵袭相关 EtMIC 基因表达的下调来介导的。
